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ARS Home » Southeast Area » Mississippi State, Mississippi » Crop Science Research Laboratory » Genetics and Sustainable Agriculture Research » Research » Publications at this Location » Publication #226028
Title: Use of fiber and fuzz mutants to detect QTL for yield components, seed, and fiber traits of Upland cotton

Author
item AN, CHUANFU - MISSISSIPPI STATE UNIV
item Jenkins, Johnie
item WU, JIXIANG - MISSISSIPPI STATE UNIV
item GUO, YUFANG - MISSISSIPPI STATE UNIV
item McCarty, Jack

Submitted to: Euphytica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/22/2009
Publication Date: 2/1/2010
Citation: An, C., Jenkins, J.N., Wu, J., Guo, Y., McCarty Jr., J.C. 2010. Use of Fiber and Fuzz Mutants to Detect QTL for Yield Components, Seed, and Fiber Traits of Upland Cotton. Euphytica. 172:21-34.

Interpretive Summary: The purpose of this research was to detect quantitative trait loci (QTL) or molecular markers associated with yield components, fiber, and seed traits within multiple fuzzless loci genetic backgrounds. Two F2 populations developed from crossing MD17, a fuzzless-lintless genetic stock containing three fuzzless loci, N1, n2 and a postulated n3, with line 181 (fuzzless-linted) and with FM966, a fuzzy-linted cultivar, were used for molecular mapping by SSR markers. A major QTL which explains 68.3 (in the population with FM 966) and 87.1% (in the population with 181) of the phenotypic variation for lint percentage and 62.8% (in the population with 181) for lint index were detected in the vicinity of BNL3482-138 on chromosome 26. Regression analyses showed marker STV79-108, which was located to the long arm of chromosome 12 (the known location of N1 and perhaps n2 loci), also had significant association with lint percentage (R2 26.7%), lint index (R2 30.6%), embryo protein percentage (R2 15.4%), and micronaire (R2 20.0%). Two-locus epistatic interactions were also observed. Results from this research will facilitate further understanding of the complex mechanisms of yield, fiber, and seed traits of cotton.

Technical Abstract: Cotton (Gossypium spp.) is the world’s leading fiber crop and an important source of protein and oil. The purpose of this research was to detect QTL or molecular markers associated with yield components, fiber, and seed traits within multiple fuzzless loci genetic backgrounds. Two F2 populations developed from crossing MD17, a fuzzless-lintless genetic stock containing three fuzzless loci, N1, n2 and a postulated n3, with line 181 (fuzzless-linted) and with FM966, a fuzzy-linted cultivar, were used for molecular mapping by polymorphic SSR markers. Major QTL which explain 68.3 (population with FM 966) to 87.1% (population with 181) of the phenotypic variation for lint percentage and 62.8% (population with 181) for lint index were detected in the vicinity of BNL3482-138 on chromosome 26. Single marker regression analyses indicated STV79-108, which was located to the long arm of chromosome 12 (the known location of N1 and perhaps n2 loci), also had significant association with lint percentage (R2 26.7%), lint index (R2 30.6%), embryo protein percentage (R2 15.4%) and micronaire (R2 20.0%). Additional QTL and markers associated with other seed and fiber traits were detected on different chromosome locations and explained different percentages of the phenotypic variation. Two-locus epistatic interactions were also observed. Results from this research will facilitate further understanding the complex mechanisms of yield, fiber, and seed traits of cotton.