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Title: Serial analysis of gene expression in Rhipcephalus (Boophilus) microplus following organophoosphate treatment of larvae from organophosphate resistant and susceptible strains.

Author
item Saldivar, Leonel
item Guerrero, Felicito
item Bendele, Kylie
item PLEASANCE, ERIN - G.C.I. VANCOUVER CANADA
item VARHOL, RICHARD - G.C.I. VANCOUVER CANADA
item NENE, VISHVANATH - U OF MARYLAND, BALTIMORE

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: 1/8/2008
Publication Date: 5/14/2008
Citation: Guerrero, F. 2008. Serial analysis of gene expression in Rhipcephalus (Boophilus) microplus following organophosphate treatment of larvae from organophosphate resistant and susceptible strains. National Center for Biotechnology Information (NCBI). Avbailable: http://www.ncbi.nlm.nih.gov. GEO Submissions Series Record Number GSE10109 and Sample Numbers GSM255662 (OP Susceptible Munoz OP-treated), GSM255664 (OP Susceptible Munoz untreated), GSM255665 (OP Resistant San Roman OP-treated), and GSM 255666 (OP Resistant San Roman untreated).

Interpretive Summary: Organophosphate resistant and susceptible tick larvae from laboratory strains of the southern cattle tick, Rhipicephalus (Boophilus) microplus were exposed to low doses of the organophosphate (OP) acaricide, coumaphos. The technique of serial analysis of gene expression (SAGE) was used to analyze differential gene expression in response to OP treatment and to compare the responses of OP-treated and -untreated resistant and susceptible ticks. The aim was to identify specific genes which are either activated or deactiviated upon tick exposure to coumaphos. A database of gene sequences exists and is designated the B. microplus Gene Index (http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/gimain.pl?gudb=b_microplus). This database was used as a resource of DNA sequences to help determine the function and identity of genes whose abundance changed in response to acaricide exposure. Of particular interest is a gene which was identified as a cytochrome P450 EST in the Gene Index. This gene's product was more abundant in untreated OP resistant larvae compared to untreated OP susceptible larvae. This gene's product was also more abundant in OP resistant larvae treated with OP compared to OP susceptible larvae treated with OP. Cytochrome P450s form a large gene family, many of whose members are involved in detoxification of various chemicals and whose abundance increases under exposure to toxins. Because this specific P450 from . microplus appears to be more common in OP resistant or OP treated ticks, a role in pesticide resistance is possible for this gene. This data, consisting of 4 SAGE tag libraries, was submitted and published to GenBank under GEO Submissions Series Record Number GSE10109 and Sample Numbers GSM255662 (OP Susceptible Munoz OP-treated), GSM255664 (OP Susceptible Munoz untreated), GSM255665 (OP Resistant San Roman OP-treated), and GSM 255666 (OP Resistant San Roman untreated).

Technical Abstract: Organophosphate resistant and susceptible tick larvae from laboratory strains of the southern cattle tick, Rhipicephalus (Boophilus) microplus were exposed to low doses of the organophosphate (OP) acaricide, coumaphos. Serial analysis of gene expression (SAGE) was used to analyze differential gene expression in response to OP treatment and to compare the responses of OP-treated and -untreated resistant and susceptible ticks. A B. microplus Gene Index (http://www.tigr.org/tdb/tgi) was used as an EST database to identify genes which corresponded to SAGE tags whose abundance changed in response to acaricide exposure. Of particular interest is a SAGE tag which corresponds to a cytochrome P450 EST in the Gene Index which was more abundant in untreated OP resistant larvae compared to untreated OP susceptible larvae. This SAGE tag was also more abundant in OP resistant larvae treated with OP compared to OP susceptible larvae treated with OP. This data, consisting of 4 SAGE tag libraries, was submitted and published to GenBank under GEO Submissions Series Record Number GSE10109 and Sample Numbers GSM255662 (OP Susceptible Munoz OP-treated), GSM255664 (OP Susceptible Munoz untreated), GSM255665 (OP Resistant San Roman OP-treated), and GSM 255666 (OP Resistant San Roman untreated).