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Title: Microarray analysis of expressed genes from Rhipcephalus (Boophilus) microplus larvae, adult ovary, and adult female gut associated with Babesia bovis infection

Author
item Saldivar, Leonel
item Guerrero, Felicito
item Bendele, Kylie
item BRAYTON, KELLY - WASHINGTON STATE, PULLMAN
item Scoles, Glen

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: 3/13/2008
Publication Date: 12/31/2008
Citation: Saldivar, L., Guerrero, F., Bendele, K.G., Brayton, K.A., Scoles, G.A. 2008. Microarray analysis of expressed genes from Rhipcephalus (Boophilus) microplus larvae, adult ovary, and adult female gut associated with Babesia bovis infection. National Center for Biotechnology Information (NCBI). Available: http://www.ncbi.nlm.nih.gov. GEO Submissions Series Record Number GSE10816, Sample Numbers GSM273028-273051.

Interpretive Summary: Rhipcephalus (Boophilus) microplus ticks are vectors of Babesia bovis, the protozoan causative agent of cattle fever, a disease which is responsible for significant production losses to cattle producers in much of Africa, Central and South America and Australia. We initiated a study of differential gene expression in Rhipicephalus (Boophilus) microplus following infection by Babesia bovis in an attempt to identify tick genes which might be critical to successful pathogen infection. These critical genes might be candidates for development of anti-Babesia control technologies. We utilized a microarray which contained over 13,000 R. microplus gene probes which allowed the isolation of genes preferentially activated following the adult tick's infection with Babesia bovis. We removed the gut and ovary from engorged Babesia bovis-infected fertile R. microplus females and control uninfected females and probed replicate microarrays. A subset of the infected females were allowed to produce eggs and larvae from these Babesia bovis-infected eggs and control uninfected eggs were used to probe microarrays to identify genes over-expressed in Babesia bovis-infected R. microplus larvae compared to uninfected controls. Thus, we have used R. microplus microarrays to identify genes which are over-expressed in Babesia bovis-infected larvae, ovaries and guts compared to uninfected controls. The microarray datasets have been submitted and published under GEO Submissions Series Record Number GSE10816, Sample Numbers GSM273028-273051.

Technical Abstract: Boophilus ticks are vectors of Babesia bovis, the protozoan causative agent of cattle fever, a disease which is responsible for significant production losses to cattle producers in much of Africa, Central and South America and Australia. We utilized R. microplus microarrays, which contained over 13,000 R. microplus expressed gene probes, to obtain DNA sequences from genes over-expressed in Babesis bovis-infected B. microplus larvae, adult ovary and adult female gut compared to uninfected controls. The microarray datasets have been submitted and published under GEO Submissions Series Record Number GSE10816, Sample Numbers GSM273028-273051.