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ARS Home » Midwest Area » Lexington, Kentucky » Forage-animal Production Research » Research » Publications at this Location » Publication #228827
Title: Clover Biotechnology Research at FAPRU

Author
item THAKARE, D - UNIVERSITY OF KENTUCKY
item Dinkins, Randy
item KUMUDINI, S - UNIVERSITY OF KENTUCKY

Submitted to: North American Alfalfa Improvement Conference
Publication Type: Other
Publication Acceptance Date: 5/30/2008
Publication Date: 6/1/2008
Citation: Thakare, D., Dinkins, R.D., Kumudini, S. 2008. Clover Biotechnology Research at FAPRU. North American Alfalfa Improvement Conference. This was a report only. No actual publication exists.

Interpretive Summary: Randy Dinkins (USDA-ARS-FAPRU) is conducting research to determine the utility of using the Medicago Affymetrix Genechip for use with red clover (Trifolium pretense). The Medicago Affymetrix Genechip contains approximately 51,000 probe sets that are derived from Medicago truncatula, 1,800 from Medicago sativa and 8,300 from Sinorhizobium meliloti. A microarray experiment was run in triplicate using RNA isolated from the first trifoliate leaves from red clover seedlings and compared to similar samples of M.truncatula. Variation between replicates of the plant derived sequences for each species was very low, however gene expression levels varied substantially between the two species with many of the genes (probe sets) called absent in the red clover samples. This may be due to sequence differences in the 3’ end of the red clover mRNAs. Analysis is ongoing to determine more precisely the differences in gene expression for the two species observed on the array.

Technical Abstract: Randy Dinkins (USDA-ARS-FAPRU) is conducting research to determine the utility of using the Medicago Affymetrix Genechip for use with red clover (Trifolium pretense). The Medicago Affymetrix Genechip contains approximately 51,000 probe sets that are derived from Medicago truncatula, 1,800 from Medicago sativa and 8,300 from Sinorhizobium meliloti. A microarray experiment was run in triplicate using RNA isolated from the first trifoliate leaves from red clover seedlings and compared to similar samples of M.truncatula. Variation between replicates of the plant derived sequences for each species was very low, however gene expression levels varied substantially between the two species with many of the genes (probe sets) called absent in the red clover samples. This may be due to sequence differences in the 3’ end of the red clover mRNAs. Analysis is ongoing to determine more precisely the differences in gene expression for the two species observed on the array.