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Title: Identification of genomic polymorphisms in upstream elements of the bovine CYP3A28 gene

Author
item MURPHY, K - UNIV. OF ARKANSAS
item REITER, S - UNIV. OF ARKANSAS
item BROWN, MICHAEL
item OKIMOTO, R - COBB-VANTRESS, ARKANSAS
item BROWN, JR., H - UNIV. OF ARKANSAS
item ROSENKRANS, JR., C - UNIV. OF ARKANSAS

Submitted to: American Society of Animal Science Southern Section Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 12/10/2007
Publication Date: 2/5/2008
Citation: Murphy, K., Reiter, S., Brown, M.A., Okimoto, R., Brown, Jr., H., Rosenkrans, Jr., C. 2008. Identification of genomic polymorphisms in upstream elements of the bovine CYP3A28 gene [abstract]. American Society of Animal Science Southern Section Meeting, February 4-5, 2008, Dallas, TX. p. 36. Available on-line: http://www.asas.org/southern/meetings_past.asp.

Interpretive Summary: Abstract Only.

Technical Abstract: The cytochrome P450 (CYP) enzyme family consists of a group of heme-containing monooxygenases that participate in metabolism and phase I detoxification. Previously, our group reported that the coding sequence for the CYP3A28 gene in cattle was polymorphic and related to cattle performance on toxic tall fescue. Our objective was to evaluate the 5'-upstream region of the CYP3A28 gene for single nucleotide polymorphisms (SNP). Genomic DNA was prepared from purebred Brahman (n = 14) and purebred Angus (n = 8) cows. Specific primers for bovine CYP3A28 upstream region were developed and resulted in a 515 base amplicon. Each amplification product was sequenced in both the forward and reverse orientation. Seven locations were found to be polymorphic. The SNPs were labeled with respect to their relative distance from initiation of exon one. Frequency of SNP distribution, as affected by breed (Angus vs. Brahman), was determined by Chi-square analyses. Frequency of SNPs t-318c, t-113a, a06g, and t21c was affected (P < 0.03) by breed. All cows that were homozygous cytosine, homozygous adenine, homozygous guanine, or homozygous cytosine for t-318c, t-113a, a06g, and t21c, respectively, were Brahman. Cows that were heterozygous for those locations also were Brahman. Frequency of three additional sites (c-189t, t-78g, and g17a) was not affected (P > 0.09) by breed. Our results indicate that the 5'-upstream region of the bovine CYP3A28 gene was polymorphic and frequency was affected by cattle breed. Future work will evaluate the relationship of these polymorphisms and cattle susceptibility to ergot alkaloid poisoning.