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ARS Home » Southeast Area » Stoneville, Mississippi » Warmwater Aquaculture Research Unit » Research » Publications at this Location » Publication #230626
Title: Growth of Piscirickettsia salmonis on Enriched Blood Agar

Author
item MAUEL, M - MISS. STATE UNIVERSITY
item WARE, C - MISS. STATE UNIVERSITY
item SMITH, P - MISS. STATE UNIVERSITY

Submitted to: Journal of Veterinary Diagnostic Investigation
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/1/2008
Publication Date: 3/1/2008
Citation: Mauel, M.J., Ware, C., Smith, P. 2008. Growth of Piscirickettsia salmonis on Enriched Blood Agar. Journal of Veterinary Diagnostic Investigation. 20(2):213-214.

Interpretive Summary: Research on Piscirickettsia salmonis has been hampered by the necessity and cost of isolating and growing the bacteria in cell culture. Enriched blood agar has now been demonstrated to support the growth of P. salmonis which will facility the development of vaccines and reduce the cost of research.

Technical Abstract: Piscirickettsia salmonis is the etiologic agent of piscirickettsiosis, an economically significant disease of fish. Isolation of P. salmonis by culturing on fish cell lines has been the standard technique since the initial isolation of the organism. The ability to grow P. salmonis on artificial media would relieve facilities of the cost of maintaining cell lines, permit isolation at fish culture sites with fewer contamination problems, and allow easier transport of isolates to diagnostic facilities for confirmation assays. This report describes the successful culture of P. salmonis on enriched blood agar.