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Title: Au/Si nanorod-based biosensor for food pathogen detection

Author
item Park, Bosoon
item FU, JUNSUE - University Of Georgia
item ZHAO, YIPING - University Of Georgia
item Siragusa, Gregory
item CHO, YONG-JIN - Korea Food Research Institute

Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 5/15/2007
Publication Date: 10/21/2007
Citation: Park, B., Fu, J., Zhao, Y., Siragusa, G.R., Cho, Y. 2007. Au/Si nanorod-based biosensor for food pathogen detection. Proceedings of 36th UJNR Food & Agri. Panel Meeting, Tsukuba, Japan. p35-38.

Interpretive Summary: Interpretive Summary Salmonella is one of the major causes of bacterial gastroenteritis in humans and a source of many food-related outbreaks. Detection of Salmonella is an important task in microbiological analysis to control food safety. Different methods have been developed in order to detect the Salmonella pathogen. However, the biggest challenges are the detection speed and sensitivity. Nanotechnology based biosensor has shown great potentials for protein and virus detection. Recently, among the many potential nanotechnology applications for food systems, development of nanoscale sensors for food safety and quality measurement are emerging. In this study, a novel nanorod-based biosensor for Salmonella detection was developed using gold and silicon materials for rapid detection of Salmonella with high sensitivity.

Technical Abstract: Technical Abstract Among several potentials of nanotechnology applications for food industry, development of nanoscale sensors for food safety and quality measurement are emerging. A novel biosensor for Salmonella detection was developed using Au/Si nanorods. The Si nanorods were fabricated by glancing angle deposition method and the Au was sputtered onto the Si nanorods. Alexa 488-succinimide dye was immobilized onto the annealed Si nanorods via the attachment between the dye ester and the primary amine supplied by the 3-Aminopropyltriethoxysilane (APTES). The anti-Salmonella was conjugated to Au via Dithiobis [succinimidylpropionate] (DSP) self-assembly monolayer (SAM). Due to the high aspect ratio nature of the Si nanorods, hundreds or thousands of dye molecules attached to the Si nanorods produced enhanced fluorescence signals. These biologically functionalized nanorods have been successfully used to detect Salmonella. This new nanoscale biosensor will be of great significance in the food safety and quality.