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ARS Home » Midwest Area » Urbana, Illinois » Soybean/maize Germplasm, Pathology, and Genetics Research » Research » Publications at this Location » Publication #231112
Title: Preliminary Analysis of Soybean Gene Expression Response to a Bradyrhizobium japonicum Type III Secretion System Mutant

Author
item RADWAN, OSMAN - UNIVERSITY OF ILLINOIS
item CARBAJULCA, DORIS - UNIVERSITY OF ILLINOIS
item LIBAULT, MARC - UNIVERSITY OF MISSOURI
item BRECHENMACHER, LAURANT - UNIVERSITY OF MISSOURI
item Clough, Steven

Submitted to: International Conference on Legume Genomics and Genetics
Publication Type: Abstract Only
Publication Acceptance Date: 8/20/2008
Publication Date: 12/7/2008
Citation: Radwan, O., Carbajulca, D., Libault, M., Brechenmacher, L., Clough, S.J. 2008. Preliminary Analysis of Soybean Gene Expression Response to a Bradyrhizobium japonicum Type III Secretion System Mutant [abstract]. 4th International Conference on Legume Genomics and Genetics, December 7-12, 2008, Puerto Vallarta, Mexico. 2008 CDROM.

Interpretive Summary:

Technical Abstract: Plant pathogens deliver proteinaceous effector molecules into their host via complex secretion systems, such as the type III secretion system (T3SS). Some of these T3SS effectors have been shown to function as suppressors of host defense responses. The role of the T3SS during plant interactions with symbionts is just beginning to be characterized. The symbiont, Bradyrhizobium japonicum, has a T3SS that is required for proper nodulation of soybean (2002 MPMI 15:1228-1235). Affymetrix gene chip analysis of soybean root-hair response to a T3SS mutant and wild-type B. japonicum versus mock inoculation at 6, 12, and 18 hours post treatment, identified 3051 transcripts as being differentially expressed at fdr adjust p-value of <0.01 due to overall treatment effect. Preliminary analysis of these results identified transcripts related to redox, proteases, channels, ethylene, calcium binding, and glutathione as being more abundant in root hairs challenged with the T3SS mutant than wild type.