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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Genetics and Animal Breeding » Research » Publications at this Location » Publication #231764
Title: MicroRNA Transcriptome Profiles During Swine Skeletal Muscle Development

Author
item McDaneld, Tara
item Smith, Timothy - Tim
item DOUMIT, MATTHEW - UNIV. IDAHO, MOSCOW
item Miles, Jeremy
item COUTINHO, LUIZ - UNIV. SAO PAULO, BRAZIL
item Sonstegard, Tad
item MATUKUMALLI, LAKSHMI - GEORGE MASON UNIVERSITY
item Nonneman, Danny - Dan
item Wiedmann, Ralph

Submitted to: BMC Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/10/2009
Publication Date: 5/1/2009
Citation: McDaneld, T.G., Smith, T.P., Doumit, M.E., Miles, J.R., Coutinho, L.L., Sonstegard, T.S., Matukumalli, L.K., Nonneman, D.J., Wiedmann, R.T. 2009. MicroRNA Transcriptome Profiles During Swine Skeletal Muscle Development. Biomed Central (BMC) Genomics. 10:77.

Interpretive Summary: MicroRNA (miR) are small RNA sequences that regulate abundance of gene transcripts and are involved in developmental processes of the animal. To evaluate the role of miR in skeletal muscle of swine, global microRNA abundance was measured at specific developmental stages including proliferating satellite cells, three stages of fetal growth, day-old neonate, and the adult. Twelve potential novel miR were detected. In addition, a number of known miR were detected, having a variety of abundance patterns through muscle development. These data present a complete set of profiles to evaluate miR abundance at specific stages of skeletal muscle growth in swine. Identification of these miR provides an initial group of miR that may play a vital role in skeletal muscle development and growth.

Technical Abstract: MicroRNA (miR) are a class of small RNAs that regulate gene expression by inhibiting translation of protein encoding transcripts. To evaluate the role of miR in skeletal muscle of swine, global microRNA abundance was measured at specific developmental stages including proliferating satellite cells, three stages of fetal growth, day-old neonate, and the adult. Twelve potential novel miR were detected that did not match previously reported sequences. In addition, a number of miR previously reported to be expressed in mammalian muscle were detected, having a variety of expression patterns through muscle development. Muscle-specific miR-206 was nearly absent in proliferating satellite cells in culture, but was the highest expressed miR at other time points evaluated. In addition, miR-1 was moderately expressed throughout developmental stages with highest expression in the adult. In contrast, miR-133 was moderately expressed in adult skeletal muscle and either not detectable or lowly expressed throughout fetal and neonate development. Changes in abundance of ubiquitously expressed miR were also observed. MiR-432 abundance was highest at the earliest stage of fetal development tested (d 60) and decreased throughout development to the adult. Conversely, miR-24 and miR-27 exhibited greatest expression in proliferating satellite cells and the adult, while expression of miR-368, miR-376, and miR-423-5p was greatest in the neonate. These data present a complete set of expression profiles to evaluate miR abundance at specific stages of skeletal muscle growth in swine. Identification of these miR provides an initial group of miR that may play a vital role in skeletal muscle development and growth.