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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Food Processing and Sensory Quality Research » Research » Publications at this Location » Publication #233421
Title: Epitope analysis of peanut allergen Ara h1 with oligoclonal IgM antibody from human B-lymphoblastoid cells.

Author
item NAGANAWA, YASUNORI - INST. BIOSCIENCE & TECH
item SHIMMOTO, MICHIE - TOKYO INST. MEDICAL SCI
item Maleki, Soheila
item TAKASE, MASAYUKI - NATL. FOOD RESEARCH
item SHINMOTO, HIROSHI - NATL. FOOD RESEARCH

Submitted to: Cytotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/11/2008
Publication Date: 6/1/2008
Citation: Naganawa, Y., Shimmoto, M., Maleki, S.J., Takase, M., Shinmoto, H. 2008. Epitope analysis of peanut allergen Ara h1 with oligoclonal IgM antibody from human B-lymphoblastoid cells. Cytotechnology. 9142-9143.

Interpretive Summary: To identify the location of the binding sites on a major peanut allergy by human antibodies, human immune cells were cultured. The antibodies they produced were harvested and analyzed for binding to Ara h 1, a major peanut allergen. A unique antibody binding area was identified between amino acid 171 and 230 of the allergen and was named “area c”.

Technical Abstract: To analyze epitopes of peanut allergen Ara h1, Epstein-Barr virus-transformed human peripheral oligoclonal B-cells were cultured to obtain antibodies to Ara h1. The combined reaction pattern with six oligoclonal antibodies showed there were six antibody binding areas named a to f in Ara h1. We found the novel antibody binding area named ‘‘area c’’ (171–230aa).