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Title: Insertion of a Reticuloendotheliosis virus LTR into the Marek's disease virus genome

Author
item Silva, Robert
item KIM, TAEJOONG - MICHIGAN STATE UNIVERSITY
item Mays, Jody
item Fadly, Aly

Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/11/2009
Publication Date: 7/11/2009
Citation: Silva, R.F., Kim, T., Mays, J.K., Fadly, A.M. 2009. Insertion of a Reticuloendotheliosis Virus LTR into the Marek's Disease Virus Genome [abstract]. In: 146th American Veterinary Medical Association Convention Notes, July 11-14, 2009, Seattle, Washington. Paper No. 7660.

Interpretive Summary:

Technical Abstract: Marek’s disease virus (MDV) had previously been co-cultivated in culture with Reticuloendotheliosis virus (REV). During co-cultivation, a long terminal repeat (LTR) from REV was inserted into the MDV genome. The resulting MDV, designated RM1, was attenuated but still induced severe thymic and bursal atrophy in inoculated chickens. To determine whether the LTR insertion was responsible for the altered phenotype, we isolated and cloned an REV LTR into a bacterial artificial chromosome clone of MDV. We will report on the cloning procedure and preliminary characterization of the chimeric MDV.