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Title: Single assay for simultaneous detection and differential identification of human and avian influenza virus types, subtypes, and emergent variants

Author
item METZGAR, D - NAVAL HEALTH RESEARCH CT
item MYERS, C - NAVAL HEALTH RESEARCH CT
item RUSSELL, K - NAVAL HEALTH RESEARCH CT
item FAIX, D - NAVAL HEALTH RESEARCH CT
item BLAIR, PATRICK - Naval Health Research Center
item BROWN, J - NAVAL HEALTH RESEARCH CT
item VO, SCOTT - Naval Health Research Center
item Swayne, David
item Thomas, Colleen
item STENGER, D - NAVAL RESEARCH LAB
item LIN, B - NAVAL RESEARCH LAB
item MALANOWSKI, A - NAVAL RESEARCH LAB
item WANG, Z - NAVAL RESEARCH LAB
item BLANEY, K - NAVAL RESEARCH LAB
item LONG, N - NAVAL RESEARCH LAB
item SCHNUR, J - NAVAL RESEARCH LAB
item SAAD, M - NAVAL MED RU-3, EGYPT
item BORSUK, L - TESSARAE, LLC
item LICHANSKA, A - TESSARAE, LLC
item LORENCE, M - TESSARAE, LLC
item WESLOWSKI, B - TESSARAE, LLC
item SCHAFER, K - TESSARAE, LLC
item TIBBETTS, C - TESSARAE, LLC

Submitted to: PLOS ONE
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/4/2010
Publication Date: 2/3/2010
Citation: Metzgar, D., Myers, C., Russell, K., Faix, D., Blair, P., Brown, J., Vo, S., Swayne, D.E., Thomas, C., Stenger, D., Lin, B., Malanowski, A., Wang, Z., Blaney, K., Long, N., Schnur, J., Saad, M., Borsuk, L., Lichanska, A., Lorence, M., Weslowski, B., Schafer, K., Tibbetts, C. 2010. Single assay for simultaneous detection and differential identification of human and avian influenza virus types, subtypes, and emergent variants. PLoS One. 5(2):e8995. DOI: 10.1371/journal.pone.0008995.

Interpretive Summary: Rapid and accurate detection, identification and genetic characterization are essential for effective monitoring for influenza viruses. This report describes applications of a new biotechnology method capable of simultaneous obtaining gene sequences of influenza virus and 29 other viral and bacterial pathogens that have been associated with respiratory illness. The new assay was better at detecting and subtyping influenza viruses, than other molecular methodologies. This will save time and reduce the cost for identifying respiratory pathogens.

Technical Abstract: Rapid and accurate detection, identification and genetic characterization are essential for effective surveillance and epidemiological tracking of influenza viruses. This report describes applications of a resequencing pathogen microarray (RPM) assay that is capable of simultaneous sequencing of subtypes of Type A and Type B influenza virus hemagglutinin and neuraminidase genes; selected influenza virus matrix (M), NS and PB2 genome segments; and multiple representative genes of 29 other viral and bacterial pathogens that have been associated with influenza-like illness. The RPM assay has equivalent to greater sensitivity and superior specificity for detection and subtype identification of influenza viruses, and it delivers significantly greater decision-quality information per assay than traditional serological subtyping assays or more recently adopted polymerase chain reaction (PCR)-based methodologies.