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ARS Home » Southeast Area » Dawson, Georgia » National Peanut Research Laboratory » Research » Publications at this Location » Publication #238609
Title: Comparison of ELISA and RT-PCR assays for the detection of Tomato spotted wilt virus (TSWV) infection in peanut

Author
item Dang, Phat
item ROWLAND, DIANE
item Faircloth, Wilson

Submitted to: American Peanut Research and Education Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 8/8/2009
Publication Date: 11/5/2009
Citation: Dang, P.M., Rowland, D., Faircloth, W.H. 2009. Comparison of ELISA and RT-PCR assays for the detection of Tomato spotted wilt virus (TSWV) infection in peanut. American Peanut Research and Education Society Abstracts. 36:133-137.

Interpretive Summary: Does not require.

Technical Abstract: Peanut (Arachis hypogaea L.) plants infected with Tomato spotted wilt virus (TSWV) can display a wide range of disease incidence and severity depending on the year and location. Diagnosis of TSWV infection can be accomplished using enzyme-linked immunosorbent assay (ELISA) or reverse transcription polymerase chain reaction (RT-PCR) but there has been no report of a direct comparison of the success of the two techniques in evaluating infection rates of field-grown peanut plants. We collected peanut roots from field grown plants, 76 in 2006 and 48 for 2007, and subjected these samples to both ELISA and RT-PCR to test for the presence of TSWV. Out of 124 samples, 50 (40%) vs. 57 (46%) of the samples were positive for TSWV infection by ELISA and RT-PCR respectively. However, statistical analysis showed no significant difference between testing results for TSWV infection between these two methods. This result supports the conclusion that ELISA and RT-PCR are comparable methods for detecting TSWV infection rates in field-grown peanut.