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Title: 454 pyrosequencing project identifying expressed genes from the horn fly, Haematobia irritans

Author
item Saldivar, Leonel
item Guerrero, Felicito
item DOWD, S - Research And Testing Laboratories, Llc
item DJIKENG, G - J Craig Venter Institute
item WILEY, G - University Of Oklahoma
item MACMIL, S - University Of Oklahoma
item NAJAR, F - University Of Oklahoma
item ROE, B - University Of Oklahoma

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: 9/9/2008
Publication Date: 9/9/2008
Citation: Saldivar, L., Guerrero, F., Dowd, S.E., Djikeng, G., Wiley, G., Macmil, S., Najar, F., Roe, B.A. 2008. 454 pyrosequencing project identifying expressed genes from the horn fly, Haematobia irritans. National Center for Biotechnology Information (NCBI). Available: http://www.ncbi.nlm.nih.gov. Assession number SRA001542

Interpretive Summary: The horn fly, Haematobia irritans, is a blood-feeding parasite of cattle which is a controlled with good animal husbandry practices and the application of pesticides. Pesticide resistance is a developing problem for producers and has a negative economic impact upon production. We established a large sequencing project to identify genes from the horn fly. We hope to identify genes which are involved in the development of pesticide resistance and also identify genes which might prove candidate targets for the design of novel control technologies. In this project, we used 454 pyrosequencing techniques to sequence 73,512, 100,603, 71,550, and 85,769 expressed genes from the egg, first instar larvae, adult male, and adult female lifestages of the horn fly. These sequences have been submitted and published under accession number SRA001542 in the National Center for Biotechnology Information Public Database.

Technical Abstract: We used an EST approach to initiate a study of the genome of the horn fly, Haematobia irritans and have used 454 pyrosequencing techniques to sequence 73,512, 100,603, 71,550, and 85,769 expressed genes from the egg, first instar larvae, adult male, and adult female lifestages of the horn fly. cDNA was synthesized from eggs, first instar larvae, adult males, and adult females from a field population of horn flies. These sequences were used to search publicly accessible databases to identify sequence similarity to known genes and assign putative function to the horn fly genes. The unassembled sequences have been submitted and published under accession number SRA001542 in the National Center for Biotechnology Information Public Database.