Author
![]() |
SCHILLER, IRENE - PRIONICS AG |
![]() |
SCHRODER, B - PRIONICS AG |
![]() |
VORDERMEIER, H - VETERINARY LABORATORIES AGENCY (VLA) |
![]() |
WATERS, WADE |
![]() |
Palmer, Mitchell |
![]() |
Thacker, Tyler |
![]() |
WHELAN, ADAM - VETERINARY LABORATORIES AGENCY (VLA) |
![]() |
HARDEGGER, ROLAND - PRIONICS AG |
![]() |
MARG-HAUFE, BEATRICE - PRIONICS AG |
![]() |
RAEBER, ALEX - PRIONICS AG |
![]() |
OESCH, BRUNO - PRIONICS AG |
Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 8/25/2009 Publication Date: 8/25/2009 Citation: Schiller, I., Schroder, B., Vordermeier, H.M., Waters, W.R., Palmer, M.V., Thacker, T.C., Whelan, A., Hardegger, R., Marg-Haufe, B., Raeber, A., Oesch, B. 2009. Comparison of Tuberculin Activity in the Interferon-gamma Assay for the Diagnosis of Bovine Tuberculosis [abstract]. Interpretive Summary: Technical Abstract: Cattle infected with bovine tuberculosis still represent a serious regulatory and health concern in a variety of countries. Early diagnosis using the in vitro interferon gamma (IFN-gamma) assay has been applied for more than a decade. Briefly, IFN-gamma responses in whole blood cultures stimulated with purified protein derivatives (PPDs) from Mycobacterium bovis (PPD-B) or M. avium (PPD-A) are quantitated. The production of a higher amount of IFN-gamma in cultures stimulated with PPD-B in comparison to PPD-A is indicative of an M. bovis infection. In the present study, different sources and concentrations of PPDs were compared with regard to diagnostic reliability in experimentally- and naturally-infected animals. Significant differences (P < 0.05) between sources and concentrations of PPD for stimulation were detected, indicating the need for standardization of PPDs used in the IFN-gamma assay. Additionally, we propose a tool named Relative Potency 30 (RP30) that allows rapid comparison of batches and sources of PPDs. |