Skip to main content
ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Publications at this Location » Publication #248316
Title: Genetic Maps of Stem Rust Resistance Gene Sr13 in Tetraploid Wheat

Author
item Simons, Kristin
item ABATE, ZEWDIE - University Of California
item Chao, Shiaoman
item ZHANG, WENJUN - University Of California
item ROUSE, MATT - University Of Minnesota
item Jin, Yue
item DUBCOVSKY, JORGE - University Of California

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/4/2009
Publication Date: 1/9/2010
Citation: Simons, K.J., Abate, Z., Chao, S., Zhang, W., Rouse, M., Jin, Y., Dubcovsky, J. 2010. Genetic Maps of Stem Rust Resistance Gene Sr13 in Tetraploid Wheat. Proceedings of Plant and Animal Genome Conference. p. 302, San Diego, CA Jan 9-13, 2010.

Interpretive Summary: Wheat stem rust caused by Puccinia graminis f. sp. tritici, can cause significant crop loss. The development of resistant cultivars has been the most cost effective means of protecting wheat from rust epidemics. Wheat breeders have deployed resistance genes both individually and by combining multiple resistant genes in cultivars with the hope to extend the resistance durability. A new race of stem rust, TTKSK (Ug99), was discovered in Uganda in 1999. This race renders most of the resistance genes currently deployed ineffective, and is rapidly spreading to other regions of the world. It is therefore important to identify, locate resistance genes on wheat chromosomes, and deploy resistance genes that are still effective against TTKSK. One of these resistance genes, Sr13, was previously assigned to the long arm of chromosome 6A, but its precise map location is not known. We present here the genetic map locations of Sr13 in relation to molecular DNA markers using three mapping populations developed in durum wheat genetic background segregating for the resistance gene. The first population was from the cross between Kofa and UC1113, and the resistance gene was contributed by Kofa. The second and third populations include crosses between Mindum and Medora or Sceptre. In these last two populations the resistance was contributed by Medora and Sceptre. The genetic mapping results based on these three populations showed that the Sr13 gene was located at the similar location, suggesting that these durum lines carry the same resistance gene. These maps will be the foundation for developing diagnostic markers, for high-density mapping, and for the eventual position cloning of Sr13gene.

Technical Abstract: Wheat stem rust caused by Puccinia graminis f. sp. tritici, can cause significant crop loss. To avoid these losses breeders have deployed resistance genes both individually and in combined pyramids with the hope to extend the resistance durability. A new race of stem rust, TTKSK (Ug99), was discovered in Uganda in 1999. This race is virulent on most of the resistance genes currently deployed and is rapidly spreading to other regions of the world. It is therefore important to identify, map, and deploy resistance genes that are still effective against TTKSK. One of these resistance genes, Sr13, was previously assigned to the long arm of chromosome 6A but its precise map location is not known. We present here genetic maps of Sr13 developed from three tetraploid wheat (T. turgidum ssp. durum) mapping populations. The first population includes 93 SSD lines from the cross between durum lines Kofa and UC1113, and the resistance gene was contributed by Kofa and was mapped on the long arm of chromosome 6A between markers Xwmc580 and Xdupw167. The second and third populations include crosses between Mindum and Medora (97 F2 lines) or Sceptre (80 F2 lines). In these last two populations the resistance was contributed by Medora and Sceptre, and was mapped in a similar location as in the Kofa x UC1113. These results suggest that these durum lines carry the same resistance gene. Based on its chromosome location this gene is most likely Sr13. These maps will be the foundation for developing diagnostic markers, for high-density mapping, and for the eventual position cloning of Sr13.