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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Produce Safety and Microbiology Research » Research » Publications at this Location » Publication #249356
Title: Top-down proteomic identification of protein biomarkers of food-borne pathogens using MALDI-TOF-TOF-MS/MS

Author
item Fagerquist, Clifton - Keith
item Sultan, Omar

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 3/7/2011
Publication Date: 5/1/2012
Citation: Fagerquist, C.K., Sultan, O. 2012. Top-down proteomic identification of protein biomarkers of food-borne pathogens using MALDI-TOF-TOF-MS/MS. Lee, M.S. Editor. Mass Spectrometry Handbook. New Jersey:John Wiley & Sons. p. 559-573.

Interpretive Summary: This chapter describes a step-by-step protocol and discussion of top-down proteomic identification of protein biomarkers of food-borne pathogens using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-TOF-MS/MS) and web-based software developed in the Produce Safety & Microbiology Research Unit at the Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture.

Technical Abstract: This chapter describes a step-by-step protocol and discussion of top-down proteomic identification of protein biomarkers of food-borne pathogens using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-TOF-MS/MS) and web-based software developed in the Produce Safety & Microbiology Research Unit at the Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture.