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Title: Identification of molecular markers associated with low chill/heat tolerance in raspberry

Author
item VASANTHAIAH, H - Florida A & M University
item KAMBIRANDA, D - Florida A & M University
item UMAR, G - Florida A & M University
item BASHA, S - Florida A & M University
item PHILL, B - Florida A & M University
item Hunter, Wayne

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 12/14/2009
Publication Date: 1/9/2010
Citation: Vasanthaiah, H., Kambiranda, D., Umar, G., Basha, S.M., Phill, B.R., Hunter, W.B. 2010. Identification of molecular markers associated with low chill/heat tolerance in raspberry [abstract]. Plant and Animal Genome XVIII Conference, January 9-13, 2010, San Diego, California. p. 18.

Interpretive Summary:

Technical Abstract: New genetic markers were developed which are linked to cold and heat tolerant raspberries. Raspberry is a cool season crop, and as such, cannot tolerate the high temperatures of the South during the normal growing season. Expanding the commercial growth range of raspberry production to the southern United States is undoubtedly one of the most challenging tasks that any plant breeder can face. With this in view, a study was conducted to develop molecular markers for selecting low chill/ heat tolerant raspberry cultivars that are adaptive to southern environment. We selected breeding lines coupled with germplasm collected from the National Clonal Repository (USDA-ARS) in Corvallis, Oregon showing varying degrees of heat/cold tolerance. Plant DNA was isolated from leaf samples and analyzed. DNA typing was carried out using RAPD assay. Of the 100 primers screened, 10 primers yielded 142 usable DNA bands. Pairwise comparisons of unique and shared amplification products were used to construct phylogenetic trees showing relatedness which can be used to enhance breeding crosses to identify chill-tolerant raspberries. Several polymorphic bands ranging between 400 to 600 bases in length were associated with low chill/heat-loving type raspberry genotypes. Additional studies are in progress to develop specific DNA/gene markers for marker assisted selection which will aid in identifying low-chill/high temperature fruit set raspberry genotypes for successful breeding and progeny selection. This research was supported by USDA/CBG.