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Submitted to: Southern Nursery Association Research Conference
Publication Type: Proceedings Publication Acceptance Date: 9/20/2009 Publication Date: 2/12/2010 Citation: Sakhanokho, H.F. 2010. An Efficient In Vitro Regeneration System for Ornamental Ginger (Hedychium spp.). Southern Nursery Association Research Conference. vol 55 pags 298-301. Interpretive Summary: In vitro regeneration systems are routinely used for commercial mass propagation of plants or production of disease-free planting stocks. An improved and efficient regeneration protocol was established for Hedychium (ornamental ginger) via somatic embryogenesis. Eleven species and 9 cultivars of Hedychium were used in this study. Production of plants through somatic embryos often depends on the plant species or genotype. However, all the species and cultivars tested produced mature normal plants, highlighting the efficiency of the regeneration system used in this study for the Hedychium genus. Production of mature plants from callus was achieved in 3-4 months, depending on Hedychium genotype. The plantlets formed were easily acclimatized in a growth room before transfer to the greenhouse. This regeneration system can be used for either commercial mass propagation or in vitro genetic manipulation for selection and gene transfer of Hedychium species and cultivars. Technical Abstract: An improved and efficient regeneration protocol was established for Hedychium via somatic embryogenesis. The plant material used consisted of 11 species and 9 cultivars of Hedychium. The explants consisted of young leaves taken from lateral or terminal shoots of mature greenhouse grown plants. These explants were disinfested by washing under running tap water for five minutes, followed by soaking in 100 ml of 10% bleach solution to which a drop of Tween 20® was added and agitated for 10 minutes. After the disinfestation step, the explants were transferred to a callus initiation medium consisting of Murashige and Skoog (MS) macronutrients and micronutrients and B5 vitamins supplemented with 9.05 µM 2,4-D, 0.6 µM TDZ, 8.9 µM BA, 20 g l-1 sucrose, 0.2 g l-1 myo-inositol, 1 g l-1 casein hydrolysate, 1 mg l-1 thiamine, 0.75 g l-1 MgCl2, and 2 g l-1 Gelrite. The induced callus was transferred to a medium similar to the medium described above but without 2,4-D. Somatic embryos were initiated in 2-3 months, and plantlets derived from these somatic embryos were transferred to an MS basal medium supplemented with 20 g l-1 sucrose, 0.75 g l-1 MgCl2, and 2 g l-1 Gelrite with no growth regulators. The plantlets formed were easily acclimatized. Production of mature plants from callus can be accomplished in 3-4 months. |
