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Title: Diagnosis of Marek's Disease From a Japanese Quail (Coturnix Japonica) Using Paraffin-embedded Liver

Author
item Dunn, John
item SOUTHARD, TERESA - Johns Hopkins University
item COOPER, COREEN - Johns Hopkins University
item KIUPEL, MATTI - Michigan State University
item Witter, Richard

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: 8/1/2010
Publication Date: 8/1/2010
Citation: Dunn, J.R., Southard, T., Cooper, C., Kiupel, M., Witter, R.L. 2010. Diagnosis of Marek's Disease From a Japanese Quail (Coturnix Japonica) Using Paraffin-embedded Liver [abstract]. In: American Association of Avian Pathologists Symposium and Scientific Program, August 1-4, 2010, Atlanta, Georgia. Paper No. 9389.

Interpretive Summary:

Technical Abstract: A single paraffin-embedded liver section was submitted from a research flock of Japanese quail that had revealed focal infiltrations of immature lymphocytes within multiple visceral organs. Tumor cells were characterized as T-cells positive for Marek's disease virus (MDV) pp38 antigen by IHC dual staining. Confirmation of MDV infection by PCR from DNA isolated from the paraffin-block provided supportive evidence, although only successfully with PCR products <1.41 kb in length, likely due to DNA damage caused by fixation. This case report provides an example of validation for tumor virus advanced diagnostic techniques using a non-ideal sample type.