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Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 4/22/2010 Publication Date: 7/13/2010 Citation: Chen, G. 2010. Fluorometer with a quartz-rod waveguide-integrating sphere configuration to measure evanescent-field luminescence [Abstract]. Symposium on Luminescence Spectrometry. Interpretive Summary: Technical Abstract: A fluorometer was designed to measure evanescent-field luminescence. A quartz-rod waveguide (d = 2 mm) was installed coaxally inside a cylindrical flow-through cell (id = 2.3 mm, od = 6.3 mm, l = 116 mm). An excitation beam from a UV LED or a miniature xenon flashlamp was focused by a ball lens and launched into the waveguide. An integrating sphere (D = 105 mm) surrounded the waveguide-cell assembly to collect the luminescence signal originated from the evanescent field. After multiple diffusive reflections, the signal was finally coupled to a photomultiplier tube by a short waveguide (d = 5 mm) embedded on the wall perpendicular to the cell axis. This configuration improved collection efficiency leading to enhanced sensitivity. This fluorometer was operated in a flow-injection analysis mode under the control of a custom LabVIEW program. Its application included analysis of several veterinary drug residues in foods based on steady-state fluorescence or lanthanide-sensitized luminescence. The optical characteristics and analytical performance of this configuration were discussed and compared with those of other geometries in which a tapered or planar waveguide was used as the sensing element. |
