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Submitted to: Phytopathology
Publication Type: Abstract Only Publication Acceptance Date: 5/10/2010 Publication Date: 6/1/2010 Citation: Rogers, E.E. 2010. Arabidopsis Thaliana Ecotypes With Differential Susceptibility To The Bacterial Pathogen Xylella fastidiosa. Phytopathology. 100(6):S110. Interpretive Summary: Technical Abstract: Pierce’s disease of grapes and almond leaf scorch are devastating diseases caused by the bacterium Xylella fastidiosa (Xf). To date, progress in determining the mechanisms of host plant susceptibility, tolerance or resistance has been slow, due in large part to the long generation time and limited available genetic resources for grape, almond and other known hosts of Xf. The long generation time and limited genetic resources for Xylella fastidiosa compound the problem. The model plant Arabidopsis thaliana is an ideal system for rapid progress in genetic and pathological studies. There are many publically available genetic resources for Arabidopsis and it has a short generation time. Arabidopsis has been evaluated as a model host for Xf. A pin-prick inoculation method has been developed. Following infection, Xf can be detected by microscopy and PCR. Xf has also been re-isolated from infected Arabidopsis tissue. Timcourses following Xf growth have revealed Arabidopsis ecotypes with differing susceptibility to infection. The genetic inheritance of these differences is being investigated. Additionally, differences in gene expression between ecotypes following Xf infection will be presented. |
