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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Livestock Issues Research » Research » Publications at this Location » Publication #252166
Title: Arginine supplementation does not alter nitrogen metabolism of beef steers during a lipopolysaccharide challenge

Author
item CARTER, BOONE - New Mexico State University
item LOEST, CLINT - New Mexico State University
item GILLIAM, G - New Mexico State University
item GRAHAM, B - New Mexico State University
item Carroll, Jeffery
item Collier, Chad
item HALLFORD, DENNIS - New Mexico State University

Submitted to: Journal of Animal Science Supplement
Publication Type: Proceedings
Publication Acceptance Date: 4/15/2010
Publication Date: 8/5/2010
Citation: Carter, B., Loest, C., Gilliam, G., Graham, B., Carroll, J.A., Collier, C.T., Hallford, D. 2010. Arginine supplementation does not alter nitrogen metabolism of beef steers during a lipopolysaccharide challenge [abstract]. Journal of Animal Science. 88:7(E-Suppl. 2).

Interpretive Summary:

Technical Abstract: Demand for arginine (Arg) is reported to increase during immune challenges. This study evaluated effects of lipopolysaccharide (LPS) and abomasal Arg infusion on nitrogen (N) metabolism and immune response of 20 ruminally cannulated steers (369 ± 46 kg BW) in a randomized block design. Each block consisted of a 14-d adaptation, 1-d blood collection, and 5-d fecal and urine collection. Steers were fed a diet (12.9% CP, 0.99 Mcal/kg NEg) at 1.5% BW. Treatments (2 × 2 factorial) were AA solutions with no Arg (-ARG) or 10 g/d Arg (+ARG), and sterile saline with no LPS (-LPS) or 1 microgram LPS (+LPS; E. coli 055:B5) per kg BW. The AA solutions were abomasally infused (720 mL/d) from d 7 to 20; LPS solutions (100 mL) were intravenously infused (1 mL/min) on d 15. Rectal temperature (RT) and blood samples were collected 0, 2, 4, 8, 12, and 24 h after LPS infusion on d 15. No LPS × ARG × h or LPS × ARG interactions occurred (P > 0.18). Cortisol, IL-6, and RT were greater (LPS × h, P < 0.01) for +LPS vs -LPS at 2, 4 (peak), 8 and 12 h (cortisol, IL-6). Tumor necrosis factor-alpha was greater at 2 h, and haptoglobin was greater at 24 h in +LPS vs -LPS steers (LPS × h, P < 0.01). Plasma Met, Leu, Val, Gln, and Orn of +LPS vs -LPS steers were greater (Met, Leu) or not different (Val, Gln, Orn) at 0 h, not different at 2 and 4 h, lower at 8 (all) and 12 h (Met, Val, Gln, Orn), and either not different (Met, Val, Orn) or greater (Leu, Gln) at 24 h (LPS × h, P < 0.01). Plasma Thr, Ser, Asp, Asn, and Glu were lower (LPS × h, P