Title: Chapter II. Determination of Sperm Concentration; Section 3. Photometer Procedure
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Submitted to: Book Chapter
Publication Type: Book / Chapter Publication Acceptance Date: 3/2/2010 Publication Date: 3/16/2010 Citation: Long, J.A. 2010. Chapter II. Determination of sperm concentration. Section 3. Photometer procedure. In: Bakst, M.R., Long, J.A., editors. Techniques for Semen Evaluation, Semen Storage, and Fertility Determination. 2nd edition. St. Paul, MN: The Midwest Poultry Federation. p. 23-25. Interpretive Summary: A photometer provides an indirect estimate of the sperm concentration by measuring the optical density of a highly diluted semen sample. The optical density is directly proportional to the concentration of sperm in the sample. Before reading any samples, it is necessary to zero the machine using a "blank sample", a tube filled only with 3% sodium citrate). The number of sperm in the semen sample is determined by comparing the sample’s optical density to a standard curve or using the line equation supplied by the manufacturer for the photometer. Technical Abstract: N/A |
