Author
ROGAS, P - University Of Talca | |
SANDOVAL, C - University Of Talca | |
CALIGARI, P D - University Of Talca | |
Martin, Robert |
Submitted to: Phytopathology
Publication Type: Abstract Only Publication Acceptance Date: 3/24/2010 Publication Date: 6/3/2010 Citation: Rogas, P., Sandoval, C., Caligari, P.S., Martin, R.R. 2010. Assessment of Strawberry mild yellow edge virus infection in different ecotypes of the Chilean native strawberry Fragaria chiloensis (L.) Duch.. Phytopathology. 100:S110. Interpretive Summary: Technical Abstract: Fragaria chiloensis ssp chiloensis (L.) Duch, is distributed naturally in Chile. Two botanical forms have been described: the white-fruited chiloensis, which is cultivated in coastal mountains between latitudes 35° and 39°S, and the small-red fruited patagonica, which grows widely between latitudes 35°and 47°S. Fragaria chiloensis, the mother of the current commercial strawberry, produces berries with unique quality characters and has the potential to be used as a commercial berry. However, their yields can be severely affected by viral diseases. Aphid-borne viruses have been found in wild and cultivated F. chiloensis, but these plants do not show symptoms, and the defense response mechanism in this species is unknown. The objective of this work was to study the development of Strawberry mild yello edge virus infection in different ecotypes of F. chiloensis. A SMYEV isolate from Chile was used to graft-inoculate three-month-old healthy plants and a real-time PCR detection method was used for detecting the SMYEV RNA using CP specific primers. Our results showed virus detection at three days post inoculation (p.i.) in most chiloensis form ecotypes, whereas in the patagonica form the virus was not detected until six to eight weeks p.i. This suggests a differential response to SMYEV infection in the different genetic backgrounds. PR was supported by CONICYT and UTAL fellowships. |