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Title: Determining the oleic/linoleic acid ratio in a single peanut seed: a comparison of two methods

Author
item Chamberlin, Kelly
item Melouk, Hassan
item MADDEN, ROBIN - Oklahoma State University
item DILWITH, JACK - Oklahoma State University
item BANNORE, YOGITA - Oklahoma State University
item EL RASSI, ZIAD - Oklahoma State University
item PAYTON, M - Oklahoma State University

Submitted to: American Peanut Research and Education Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2010
Publication Date: 10/1/2010
Citation: Chamberlin, K.D., Melouk, H.A., Madden, R.D., Dilwith, J.W., Bannore, Y.C., El Rassi, Z., Payton, M. 2010. Determining the oleic/linoleic acid ratio in a single peanut seed: A comparison of two methods. In: 2010 Proceedings of the American Peanut Research and Education Society, July 12-15, 2010, Clearwater Beach, FL. 42:80-81.

Interpretive Summary:

Technical Abstract: Peanut varieties with high oleic/linoleic acid ratios have become preferred by the peanut industry due to their increased shelf life and improved health benefits. Many peanut breeding programs are trying to incorporate the high oleic trait into new and improved varieties and are in need of diagnostic tools to track its inheritance early in development and at the single seed level. Traditionally, gas chromatography has been used to accurately determine the properties of peanut oil, but this method generally requires modification of oil after extraction and possible destruction of the seed sample. In this study, oil was extracted from approximately 0.10 mg of peanut seed tissue taken from the distal end, leaving the seed intact for subsequent germination. Over 100 samples were processed, covering runner, Spanish and Virginia market types. Oil extractions were analyzed for oleic/linoleic acid ratio using (1) capillary electrophoresis (CE) and (2) gas chromatography (GC). Results showed that the two methods are 100% in agreement in determining whether a peanut seed is "high-oleic" or "normal" in oil content. Furthermore, the two methods are highly correlated (r = 0.96; p < 0.0001) with respect to determining the exact oleic/linoleic acid ratio from each sample. Results from this study validate the use of CE as a diagnostic tool for breeding programs to identify individual high oleic peanut seed for further testing and development.