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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #252873
Title: Genetic diversity and relationship among faba bean (Vicia faba L.) germplasm entries as revealed by TRAP markers

Author
item Kwon, Soon
item Hu, Jinguo
item Coyne, Clarice - Clare

Submitted to: Plant Genetic Resources: Characterization and Utilization
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/2/2010
Publication Date: 7/6/2010
Citation: Kwon, S.J., Hu, J., Coyne, C.J. 2010. Genetic diversity and relationship among faba bean (Vicia faba L.) germplasm entries as revealed by TRAP markers. Plant Genetic Resources: Characterization and Utilization. 8(3); 204-213.

Interpretive Summary: Faba bean is one of the deal crops for using in rotation with cereal crops since it, like other legumes, has the ability to fix atmospheric nitrogen via symbiosis with nitrogen-fixing rhizobia bacteria. We have osbserved a great range of variation in response to the cold winter conditions in Pullman and Cenrtal Ferry among the faba bean accessions in NPGS collection. This project fingerprinted the 151 faba bean accessions with the TRAP (target region amplification polymorphism) marker technique. This paper reports that TRAP is applicable to faba bean germplasm fingerprinting for 12 primer combinations (six sets of PCR) amplified a total of 221 markers, of which 122 (55.2%) were polymorphic and could discriminate all 151 entries. It was observed that there is a high level of polymorphism among the accessions with an estimated average pairwise similarity of 63.2%, ranging from 36.9 to 90.2%. Cluster analysis divided the 151 accessions into five major groups with two to 101 entries each. There seems a strong association between the molecular diversity and the geographic origin.

Technical Abstract: Target region amplification polymorphism (TRAP) markers were used to assess genetic diversity and relationship among 151 world-wide collected faba bean (Vicia faba L.) entries (137 accessions maintained at the USDA-ARS, Pullman, WA, two commercial varieties and 12 elite cultivars and advanced breeding lines obtained from W. Link of Georg-August University, Germany). Twelve primer combinations (six sets of PCR) amplified a total of 221 markers, of which 122 (55.2%) were polymorphic and could discriminate all 151 entries. A high level of polymorphism was revealed among the accessions with an estimated average pairwise similarity of 63.2%, ranging from 36.9 to 90.2%. Cluster analysis divided the 151 accessions into five major groups with two to 101 entries each. There seems a strong association between the molecular diversity and the geographic origin since all 101 accessions in Group V are originated from China and 13 of the 15 accessions in Group II were from Afghanistan. Thirty-two individual plants were sampled from two entries to assess intra-accession variation. It was found that the advanced inbred line (Hiverna/5-EP1) had very little variation (5.0%) while the original collection (PI 577746) possessed a very high amount of variation (47.1%). This is consistent with previous reports that faba been landraces have a high level of out-crossing in production fields and thus contain larger amount variation within each landrace. One implication of this observation for germplasm management is that a relatively larger population is needed in regeneration to mitigate the possible loss of genetic variation due to genetic drift.