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ARS Home » Research » Publications at this Location » Publication #253965
Title: In vitro maintenance and cryopreservation of Plant Variety Protection vouchers germplasm of Solanum tuberosum cultivars

Author
item Jenderek, Maria
item Ambruzs, Barbara - Bobbie Ambruzs
item Andre, Christina
item LAUFMAN, JULIE - Us Forest Service (FS)
item Ellis, David

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 2/16/2010
Publication Date: 6/6/2010
Citation: Jenderek, M.M., Ambruzs, B.D., Andre, C.G., Laufman, J., Ellis, D.D. 2010. In vitro maintenance and cryopreservation of Plant Variety Protection vouchers germplasm of Solanum tuberosum cultivars. Meeting Abstract. 12th International Association for Plant Biotechnology, St. Louis, Missouri, June 6-11, 2010. pp. 101.

Interpretive Summary: The USDA-ARS, National Center for Genetic Resources Preservation maintains the voucher germplasm of Plant Variety Protection (PVP) potato (S. tuberosum) cultivars registered with the U.S. Plant Variety Protection Office. The potato cultivars are deposited at the Center as tissue culture and are maintained in vitro as shoots and tubers as well as in long-term liquid nitrogen tanks as shoot tips. Voucher potato germplasm at the Center remains the property of the PVP Office and is securely maintained and kept viable for the duration of the donor’s intellectual property rights. After expiration of the PVP, the potato genetic resources are publically released through the National Plant Germplasm System. The cryopreservation procedure has evolved over years. Currently, the droplet vitrification method developed by Haeng-Hoon et al. (2006) is the widest protocol used. In the early years of the program (2000 to 2001), the post cryo shoot viability ranged from 24.7% to 39.5%; however in later years, standards were changed so that minimum of 40% was required for an accession to be considered successfully cryopreserved at the Center. Procedural modifications are made for genotypes not responding to the established protocols. Since the year 2000, 94 PVP potato accessions have been successfully placed in long-term storage and 12 additional accessions are in different stages of the cryo procedure. The PVP cultivars originated from six different countries, however the most (>43%) of the cultivars came from the U.S. donors.

Technical Abstract: The USDA-ARS, National Center for Genetic Resources Preservation maintains the voucher germplasm of Plant Variety Protection (PVP) potato (S. tuberosum) cultivars registered with the U.S. Plant Variety Protection Office. The potato cultivars are deposited at the Center as tissue culture and are maintained in vitro as shoots and tubers as well as in long-term liquid nitrogen tanks as shoot tips. Voucher potato germplasm at the Center remains the property of the PVP Office and is securely maintained and kept viable for the duration of the donor’s intellectual property rights. After expiration of the PVP, the potato genetic resources are publically released through the National Plant Germplasm System. The cryopreservation procedure has evolved over years. Currently, the droplet vitrification method developed by Haeng-Hoon et al. (2006) is the widest protocol used. In the early years of the program (2000 to 2001), the post cryo shoot viability ranged from 24.7% to 39.5%; however in later years, standards were changed so that minimum of 40% was required for an accession to be considered successfully cryopreserved at the Center. Procedural modifications are made for genotypes not responding to the established protocols. Since the year 2000, 94 PVP potato accessions have been successfully placed in long-term storage and 12 additional accessions are in different stages of the cryo procedure. The PVP cultivars originated from six different countries, however the most (>43%) of the cultivars came from the U.S. donors.