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ARS Home » Pacific West Area » Salinas, California » Crop Improvement and Protection Research » Research » Publications at this Location » Publication #255127
Title: Characterization of 22 highly polymorphic microsatellite loci in the cosmopolitan fungal plant pathogen Verticillium dahliae. Permanent Genetic Resources added to Molecular Ecology Resources

Author
item ATALLAH, ZAHI - University Of California
item MARUTHACHALAM, KARUNAKARAN - University Of California
item DAVIS, MICHAEL - University Of California
item Klosterman, Steven
item SUBBARAO, KRISHNA - University Of California

Submitted to: Molecular Ecology Resources
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/15/2009
Publication Date: 9/29/2009
Citation: Atallah, Z., Maruthachalam, K., Davis, M.R., Klosterman, S.J., Subbarao, K. 2009. Characterization of 22 highly polymorphic microsatellite loci in the cosmopolitan fungal plant pathogen Verticillium dahliae. Permanent Genetic Resources added to Molecular Ecology Resources. Molecular Ecology Resources. Molecular Ecology Resources 9:1460-1466.

Interpretive Summary: Simple sequence repeats or SSRs are useful DNA-based markers for the analyses of populations. The 22 SSRs described in this report were developed based on the newly available genomic DNA sequence of the soilborne plant pathogen Verticillium dahliae. Development of these markers is an important step in determining the genetic diversity of populations of V. dahliae.

Technical Abstract: Twenty-two microsatellite loci were characterized in the soilborne plant pathogenic fungus Verticillium dahliae by analysis of the genome sequence. All loci were polymorphic in at least two of three populations of V. dahliae tested and collected from lettuce, spinach and tomato. These loci were useful in genotyping isolates and highlighting differences in genetic diversity among the three tested populations. With this set of highly polymorphic markers, the prospective of migration, structure and evolutionary analyses is expected to provide insight into gene flow on seed and devise new strategies for development and release of resistant germplasm.