Antibiotic Resistance in Listeria Species Isolated from Catfish Fillets and Processing Environment

Authors: CHEN, B - Mississippi State University; PYLA, R - Mississippi State University; KIM, T - Mississippi State University; SILVA, J - Mississippi State University; JUNG, Y - Mississippi State University

Submitted to: Letters in Applied Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/19/2010
Publication Date: 6/1/2010
Citation: Chen, B.Y., Pyla, R., Kim, T.J., Silva, J.L., Jung, Y.S. 2010. Antibiotic Resistance in Listeria Species Isolated from Catfish Fillets and Processing Environment. Letters in Applied Microbiology. 50:662-632.

Interpretive Summary: Antibiotic (antimicrobial) resistance of foodborne pathogens is a growing concern for humans and medical personnel. This can lead to few, if any, treatments for infected humans. The prevalence of antibiotic resistance in Listeria isolates from catfish and environment was tested. It was found that in general, Listeria isolates had little if any antibiotic resistance. However, the presence of an antibiotic resistant gene in a non-pathogenic Listeria spp. Raises the possibility of future resistance buildup. Thus, it is recommended to monitor this gene and Listeria antibiotic resistance from catfish in the future.

Technical Abstract: The susceptibility of 221 Listeria spp. (86 Listeria monocytogenes, 41 Listeria innocua and 94 Listeria seeligeri-Listeria welshimeri-Listeria ivanovii) isolated from catfish fillets and processing environment to 15 antibiotics was determined. Listeria isolates were analysed by disc-diffusion assay for their resistance to 15 drugs. All isolates were resistant to cefotaxime and clindamycin but were sensitive to ampicillin, cephalothin, chloramphenicol, erythromycin, gentamycin, kanamycin, rifampin, streptomycin, sulfamethoxazole/trimethoprim and vancomycin. Unlike L. monocytogenes and L. seeligeri-L. welshimeri-L. ivanovii isolates, 22% of L. innocua isolates displayed tetracycline/oxytetracycline resistance. Screening of tet genes by PCR identified tet(M) gene in the chromosome of all tetracycline/oxytetracycline-resistant L. innocua. However, this gene was not associated with the integrase gene of Tn1545. Repetitive extragenic palindromic- and enterobacterial repetitive intergenic consensus-PCR typing methods showed no genotype-specific tetracycline resistance in the tet(M)-positive strains. Catfish fillets and processing environment were currently free of L. monocytogenes resistant to antibiotics commonly used in human listeriosis treatment. However, the presence of tet (M) gene in L. innocua raises the possibility of future acquisition of resistance by L. monocytogenes. These data will be helpful in improving background data on antibiotics resistance strains isolated from food and processing environment.