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Title: Complete genome sequence of Celery mosaic virus and its relationship to other members of the genus Potyvirus

Author
item XU, DONGLIN - South China Agricultural University
item Liu, Hsing Yeh
item LI, FAN - Yunnan Agricultural University
item Li, Ruhui

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/5/2011
Publication Date: 5/1/2011
Citation: Xu, D., Liu, H., Li, F., Li, R. 2011. Complete genome sequence of Celery mosaic virus and its relationship to other members of the genus Potyvirus. Archives of Virology. 156:917-920.

Interpretive Summary: Celery and carrots, in the family Apiaceae, are important vegetable crops. Many viruses including Celery mosaic virus (CeMV) infect these crops and cause production problems. CeMV was first reported in California in 1930s and has been a problem for celery production in many regions. The virus belongs to a viral group called potyvirus, which has thread-like particles with a single stranded RNA molecule. Although the virus has been known to cause disease for many years, its full genetic sequence had never been determined. In this study, the complete sequence of a CeMV isolate from California was determined. Analysis of the genetic information showed that the virus is most closely related to Apium virus Y, Carrot virus Y and Panax virus Y. These four viruses formed a distinct genetic cluster, indicating they may share a common ancestor in their evolution. The study provides information necessary for the proper grouping and naming of the virus. It also assists with the development of better diagnostic and management tools for these viruses and the diseases they cause in vegetable production.

Technical Abstract: The complete genomic sequence of Celery mosaic virus (CeMV) was determined to be 9999 nucleotides in length, excluding the 3’ poly(A) tail. The genome comprises a large open reading frame encoding a polyprotein of 3181 amino acid residues. Its genomic organization is typical of potyviruses, and contains conserved motifs found in the genus Potyvirus. Pairwise comparison of the polyprotein sequences shows that CeMV shares identities of 39.0-71.9% with other members of the genus Potyvirus. Phylogenetic analysis based on the polyprotein sequences indicates that CeMV is most related with Apium virus Y, and together with Panax virus Y, the three viruses form a distinct clade in the genus Potyvirus.