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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Renewable Product Technology Research » Research » Publications at this Location » Publication #265375
Title: Laccase production by diverse phylogenetic clades of Aureobasidium pullulans

Author
item Rich, Joseph
item MANITCHOTPISIT, PENNAPA - Rangsit University
item Peterson, Stephen
item Leathers, Timothy

Submitted to: Rangsit University Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/27/2011
Publication Date: 6/14/2011
Citation: Rich, J.O., Manitchotpisit, P., Peterson, S.W., Leathers, T.D. 2011. Laccase production by diverse phylogenetic clades of Aureobasidium pullulans. Rangsit Journal of Arts and Sciences. 1(1):41-47.

Interpretive Summary: Improved microbial germplasm is needed to enhance bioconversions of agricultural materials to high-value bioproducts. Genetically diverse strains of an industrially important fungus were examined for the production of an enzyme, laccase, involved in bioconversions of lignin and many other compounds. A specific genetic group of strains was identified that produces this valuable enzyme. This work will be of interest to researchers developing new uses and value-added products from agricultural commodities and byproducts.

Technical Abstract: Laccases (EC 1.10.3.2) have numerous potential industrial applications including the degradation of dyes and toxic materials. Novel sources of this enzyme would be desirable to improve activity yields and substrate specificities. In this study we tested 51 strains of A. pullulans representing 13 diverse phylogenetic clades for laccase production. Most strains grew on three different lignin-related substrates as sole carbon sources. Thirteen strains that grew well on these substrates, representing five clades, were chosen for a test of laccase production in an induction assay. Representatives of clade 5 produced laccase, indicating that this genetic group may be a promising source of novel activities.