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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #266893

Title: Gene Manipulation In Cereals

Author
item GUSTAFSON, J

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/7/2011
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Aluminum, the most abundant metal on earth, is detrimental to plant growth and agricultural production. There are about 2.5 billion hectares of acid soils high in aluminum around the world. Molecular markers linked to aluminum tolerance gene complexes in rye would be of value in marker-mediated gene introgression-breeding programs designed to improve the aluminum tolerance levels in wheat. The present study was designed to map the rye gene complex controlling aluminum tolerance, establish molecular markers flanking the gene complex, and to evaluate the potential for manipulation of the rye aluminum tolerance complex into wheat. An analysis of a rye F6 RIL population revealed three amplified fragment length polymorphism markers and one restriction fragment length polymorphism marker were identified and linked to an aluminum tolerance locus. By screening the rye map with markers developed from the utilization of rice BAC clones I constructed a high resolution linkage map of the region surrounding the aluminum tolerance locus in rye. The tightly linked molecular markers can be used to manipulate the rye aluminum tolerance locus within rye and between rye and wheat.