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Title: Identification of QTL regions and SSR markers associated with resistance to reniform nematode in Gossypium barbadense L. accession GB713

Author
item GUTIERREZ, OSMAN - Mississippi State University
item ROBINSON, ARIN - Retired ARS Employee
item JENKINS, JOHNIE - Mississippi State University
item MCCARTY, JACK - Mississippi State University
item WUBBEN, MARTIN - Mississippi State University
item CALLAHAN, FRANKLIN - Mississippi State University
item NICHOLS, ROBERT - Cotton, Inc

Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/25/2010
Publication Date: 9/16/2010
Citation: Gutierrez, O.A., Robinson, A.F., Jenkins, J.N., McCarty, J.C., Wubben, M.J., Callahan, F.E., Nichols, R.L. 2011. Identification of QTL regions and SSR markers associated with resistance to reniform nematode in Gossypium barbadense L. accession GB713. Journal of Theoretical and Applied Genetics. 122(2):271-280.

Interpretive Summary: The reniform nematode is a major pest of cotton in the U.S. The cotton accession GB713 has been identified as resistant to this nematode. To utilize this resistance in commercial cottons, methods must be found that allow breeders to identify when the resistance trait has been introduced into progeny. A known fragment of DNA that is associated with a particular trait such as resistance is known as a molecular marker. Using molecular markers, the incorporation of a trait such as resistance can be easily followed in a breeding program. We have now identified molecular markers that can be used to follow the introduction of three resistance genes from GB713 into commercial cottons. This will facilitate the development of commercial cultivars exhibiting the GB713 resistance.

Technical Abstract: The identification of molecular markers that are closely linked to gene(s) in Gossypium barbadense L. accession GB713 that confer a high level of resistance to reniform nematode (RN), Rotylenchulus reniformis Linford&Oliveira, would be very useful in cotton breeding programs. Our objectives were to determine the inheritance of RN resistance in the accession GB713, to identify SSR markers linked with RN resistance QTLs, and to map these linked markers to specific chromosomes. We grew and scored plants for RN reproduction in the P1, P2, F1, F2, BC1P1, and BC1P2 generations from the cross of GB713 X Acala Nem-X. The generation means analysis using the six generations indicated that one or more genes were involved in the RN resistance of GB713. The interspecific F2 population of 300 plants was genotyped with SSR molecular markers that covered most of the chromosomes of Upland cotton (G. hirsutum L.). Results showed two QTLs on chromosome 21 and one QTL on chromosome 18. One QTL on chromosome 21 was at map position 168.6 (LOD 28.0) flanked by SSR markers BNL 1551_162 and GH 132_199 at positions 154.2 and 177.3, respectively. A second QTL on chromosome 21 was at map position 182.7 (LOD 24.6) flanked by SSR markers BNL 4011_155 and BNL 3279_106 at positions 180.6 and 184.5, respectively. Our chromosome 21 map had 61 SSR markers covering 219 cM. One QTL with smaller genetic effects was localized to chromosome 18 at map position 39.6 (LOD 4.0) and flanked by SSR markers BNL 1721_178 and BNL 569_131 at positions 27.6 and 42.9, respectively. The two QTLs on chromosome 21 had significant additive and dominance effects, which were about equal for each QTL. The QTL on chromosome 18 showed larger additive than dominance effects. Following the precedent set by the naming of the G. longicalyx Hutchinson & Lee and G. aridum [(Rose & Standley) Skovsted] sources of resistance, we suggest the usage of Renbarb1 and Renbarb2 to designate these QTLs on chromosome 21 and Renbarb3 on chromosome 18.