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Title: Microarray analysis identified Puccinia striiformis f. sp. tritici genes involved in infection and sporulation.

Author
item HUANG, XUELING - Northwest Agriculture And Forestry University
item Chen, Xianming
item Coram, Tristan
item WANG, MEINAN - Washington State University
item KANG, SHENSHENG - Northwest Agriculture And Forestry University

Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/2010
Publication Date: 6/1/2010
Citation: Huang, X., Chen, X., Coram, T., Wang, M., Kang, S. 2010. Microarray analysis identified Puccinia striiformis f. sp. tritici genes involved in infection and sporulation.. American Phytopathological Society Annual Meeting. 100:S53.

Interpretive Summary:

Technical Abstract: Puccinia striiformis f. sp. tritici (Pst) causes stripe rust, one of the most important diseases of wheat worldwide. To identify Pst genes involved in infection and sporulation, a custom oligonucleotide Genechip was made using sequences of 442 genes selected from Pst cDNA libraries. Microarray analysis was conducted by hybridizing the Genechip with cDNA from urediniospores (Ure), germinated Ure, and Pst-infected and mock-inoculated leaves sampled at 12 h, 24 h, 48 h, 7 d, and 14 d after inoculation. The time course study identified 55 genes that were differentially induced during the infection process. Nine of the genes were induced in both Ure and the sporulation stage of infection. Genes in this group mostly have functions in carbohydrate and lipid metabolism. Six genes, including a mitochondrial ATP synthase, a copper-induced metallothionein, a differentiation-related protein Infp, and a hypothetical cell wall mannoprotein, were induced during the early infection process and therefore, likely involved in pathogenicity. Four genes, including an exo-1,3-Beta-glucanase, a chitin deacetylase, and a meiotic recombination-related protein, were induced in both early infection and sporulation stages. Thirty five genes were induced in the sporulation stage, but not in Ure, indicating that they are involved in reproduction. One gene from the haustorial library with unknown function was only induced in infected leaves.