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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #272576
Title: The transcriptome of common bean: nodules to beans

Author
item O`Rourke, Jamie
item INIGUEZ, LUIS - Universidad Nacional Autonoma De Mexico
item Bucciarelli, Bruna
item WOODY, JENNA - Iowa State University
item MCCLEAN, PHILLIP - North Dakota State University
item JACKSON, SCOTT - Purdue University
item Shoemaker, Randy
item HERNANDEZ, GEORGINA - Universidad Nacional Autonoma De Mexico
item Vance, Carroll

Submitted to: International Congress of Biochemistry and Molecular Biology
Publication Type: Abstract Only
Publication Acceptance Date: 9/21/2011
Publication Date: 11/19/2011
Citation: O'Rourke, J.A., Iniguez, L.P., Bucciarelli, B., Woody, J.L., McClean, P.E., Jackson, S.A., Shoemaker, R.C., Hernandez, G., Vance, C.P. 2011. The transcriptome of common bean: nodules to beans [abstract]. XIV National Congress of Biochemistry and Plant Molecular Biology and 7th Symposium Mexico - USA, November 29-December 2, 2011, Campeche, Mexico. p. 17.

Interpretive Summary:

Technical Abstract: Phaseolus vulgaris (common bean) is one of the most important grain legumes for direct human consumption. It comprises 50% of the grain legumes consumed worldwide and is important as a primary source of dietary protein in developing countries. We performed next generation sequencing (RNAseq) on five tissues (seeds, pods, leaves, roots, and nodules) of Phaseolus vulgaris cv. Negro Jamapa plants that were inoculated with an effective rhizobium, an ineffective rhizobium mutant strain (fix-), or were provided nitrogen via fertilization. RNA was sequenced from several types of organs at various stages of development, generating 2.1x10**9 bp of sequence data. The sequences were used in a de-novo assembly to identify expressed transcripts. Our study identified 80,463 transcript assemblies (greater than or equal to 100bp). Of these, 1,175 were expressed specifically in seeds, 827 in pods, 3,054 in roots, and 1,686 in nodules, respectively. Comparing expression profiles of transcripts in the tissues has allowed us to identify those involved in nodule, root, pod, and seed development, symbiotic nitrogen fixation, and transcripts related to the functions of the tissues analyzed. This is the first whole plant transcriptome study of Phaseolus vulgaris.