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Title: Development and characterization of expressed sequence tag-derived microsatellite markers for the wheat stripe rust fungus Puccinia striiformis f. sp. tritici

Author
item CHENG, PENG - Washington State University
item Chen, Xianming
item XU, LIANGSHENG - Washington State University
item See, Deven

Submitted to: Molecular Ecology Resources
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/13/2012
Publication Date: 5/30/2012
Citation: Cheng, P., Chen, X., Xu, L., See, D.R. 2012. Development and characterization of expressed sequence tag-derived microsatellite markers for the wheat stripe rust fungus Puccinia striiformis f. sp. tritici. Molecular Ecology Resources. 12:779-781.

Interpretive Summary: The stripe rust fungal species causes stripe rust of wheat, barley, and many grass species. Microsatellite markers are needed to study the fungal population biology. In this study, we characterized microsatellite loci based on three expressed sequence tage (EST) libraries previously developed for this fungus. By screening 3,311 unique EST sequences using a specific computer software, 46 EST sequences were selected for microsatellite motifs. Primers were designed and screened against 28 isolates of the pathogen. Thirty-four primer pairs produced stable polymorphic amplifications. Based on their genomic locations, the microsatellite loci are dispersed throughout the genome. These microsatellite markers will be useful to study the population structure and ecology of the stripe rust fungus.

Technical Abstract: Puccinia striiformis, a basidiomycete fungus, causes stripe rust of wheat, barley, and many grass species. Microsatellite markers are needed to study its population biology. In this study, we characterized microsatellite loci based on three EST libraries previously developed for this fungus. By screening 3,311 unique EST sequences using the SSRIT software, 46 EST sequences were selected for microsatellite motifs. Primers were designed and screened against 28 P. striiformis isolates. Thirty-four primer pairs produced stable polymorphic amplifications. Based on their genomic locations, the microsatellite loci are dispersed throughout the genome. These microsatellite markers will be useful to study the population structure and ecology of the stripe rust fungus.