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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Renewable Product Technology Research » Research » Publications at this Location » Publication #276528
Title: Melanin determination by high performance liquid chromatography (HPLC) for K. marxianus

Author
item Hughes, Stephen
item COX, ELBY - Bradley University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/5/2011
Publication Date: 12/5/2011
Citation: Hughes, S.R., Cox, E. 2011. Melanin determination by high performance liquid chromatography (HPLC) for K. marxianus [abstract]. p. 2.

Interpretive Summary:

Technical Abstract: Ultraviolet light (UV) mutated K. marxianus was found to turn dark brown during a growth assay. This brown color was hypothesized to be melanin overproduction influenced by the UV exposure. Cell cultures were oxidized and HPLC analyzed to determine melanin concentrations. The resulting melanin concentration of dried cell culture reconstituted at 10% by mass are below the detection limit of 63.0ppm melanin when compared to the melanin standard obtained from octopus ink.