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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Mycotoxin Prevention and Applied Microbiology Research » Research » Publications at this Location » Publication #279886
Title: Aspergillus section Versicolores: nine new species and multilocus DNA sequence based phylogeny

Author
item Peterson, Stephen
item JURJEVIC, ZELJKO - Emsl Analytical, Inc
item Horn, Bruce

Submitted to: Mycological Society of America
Publication Type: Abstract Only
Publication Acceptance Date: 7/19/2012
Publication Date: 6/21/2012
Citation: Peterson, S.W., Jurjevic, Z., Horn, B.W. 2012. Aspergillus section Versicolores: nine new species and multilocus DNA sequence based phylogeny. Mycological Society of America. 3(1):59-79.

Interpretive Summary:

Technical Abstract: ß-tubulin, calmodulin, internal transcribed spacer and partial lsu-rDNA, RNA polymerase, DNA replication licensing factor Mcm7, and pre-rRNA processing protein Tsr1 were amplified and sequenced from 62 A. versicolor clade isolates and analyzed phylogenetically using the concordance model to establish species boundaries. We used phylogeny to define species and phenotype from 10d Czapek yeast extract (CYA) cultures to describe the species. Aspergillus austroafricanus, A. creber, A. cvjetkovicii, A. fructus, A. jensenii, A. puulaauensis, A. subversicolor, A. tennesseensis and A. venenatus are described as new species and A. amoenus, A. protuberus, A. tabacinus and A. versicolor are accepted as distinct species on the basis of molecular and phenotypic differences. polymerase chain reaction (PCR) primer pairs used to detect A. versicolor in sick building syndrome studies have a positive reaction for all of the newly described species.