Author
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Edwards, Michael |
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Weiland, John |
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Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 3/24/2012 Publication Date: 7/1/2012 Citation: Edwards, M.C., Weiland, J.J. 2012. Coat protein expression strategy of oat blue dwarf virus. American Phytopathological Society, August 4-8, 2012, Providence, RI. Available: http://www.apsnet.org/meetings/Documents/2012_Meeting_Abstracts/aps12abP122.htm. Interpretive Summary: Technical Abstract: Oat blue dwarf virus (OBDV) was the first marafivirus (family Tymoviridae) to be sequenced and for which an infectious clone has been reported. Sequence data are now available for multiple marafiviruses, yet the expression strategy of these viruses remains uncharacterized. Translation experiments with OBDV suggest that a large 227 kDa polyprotein encoded by much of the genome is post-translationally processed into its functional components, in agreement with its tymoviral lineage. ORFs for the two coat proteins (CPs) of approximately 21 kDa and 24 kDa are encoded near the 3’ terminus and are coterminal with the ORF encoding this large polyprotein. Although a marafibox analogous to the tymobox is presumed to serve as a promoter for a subgenomic RNA encoding the CPs, the expression strategy for the CPs has not been thoroughly investigated. We have developed a series of point and deletion mutants in an infectious OBDV clone in an effort to dissect and analyze CP expression strategy in protoplasts. The 21 kDa (major) CP appears to be the product of direct translation of a subgenomic RNA, while the 24 kDa (minor) CP appears to be a cleavage product derived from both the polyprotein and a larger ~26 kDa precursor translated directly from a subgenomic RNA. |
