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ARS Home » Southeast Area » Tifton, Georgia » Crop Protection and Management Research » Research » Publications at this Location » Publication #280376
Title: Identification of expressed resistance gene analogs (RGA.) from peanut expressed sequence tage (EST.) and development of RGA-SSR markers

Author
item LIU, ZHANJI - University Of Georgia
item FENG, SUPING - University Of Georgia
item PANDEY, MANISH - University Of Georgia
item CULBREATH, ALBERT - University Of Georgia
item Guo, Baozhu

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 5/15/2012
Publication Date: 8/4/2012
Citation: Liu, Z., Feng, S., Pandey, M.K., Culbreath, A., Guo, B. 2012. Identification of expressed resistance gene analogs (RGA.) from peanut expressed sequence tage (EST.) and development of RGA-SSR markers [abstract]. American Phytopathological Society.

Interpretive Summary:

Technical Abstract: Cultivated peanut (Arachis hypogaea L.) is an important food and oil crop grown in more than 100 countries for providing edible oil and protein. A wide variety of pathogens including fungi, bacteria, viruses, and nematodes severely constrain peanut yield and quality. Therefore, it is very important to better understand the interaction between peanut and pathogens. Large number of peanut ESTs (225,264 ESTs by 11-11-2011) has been developed in recent years by peanut scientific community, offering resources and opportunities for gene identification and marker development. Based on the homology to typical disease resistance genes and the conservative domains, we identified 401 resistance gene analogs (RGAs), using a stringent BLAST search. These expressed RGAs are comprised of 75, 196, 89, 27, and 14 RGAs representing for NBS-LRR, protein kinase (PK), LRR-PK/TM, toxin reductase, and other domain encoding R genes, respectively. By using MISA software, 33 SSRs were identified from 28 expressed RGAs. Primers were successfully designed for 29 RGA-tagged SSRs and used for polymorphic screening among parents of two mapping populations. One of these markers, RGA121, was clearly polymorphic and segregating in two mapping populations, which was mapped on linkage group 01 along with the putative qtswv2 (Qin et al. 2012. TAG), reported to contribute to the resistance to TSWV.