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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Mycotoxin Prevention and Applied Microbiology Research » Research » Publications at this Location » Publication #282959
Title: Use of cyclodextrin-based polymer for patulin analysis in apple juice

Author
item SHIRASAWA, T - Shinshu University
item UEDA, M - Shinshu University
item Appell, Michael
item GOTO, T - Shinshu University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/3/2012
Publication Date: 10/3/2012
Citation: Shirasawa, T., Ueda, M., Appell, M.D., Goto, T. 2012. Use of cyclodextrin-based polymer for patulin analysis in apple juice. Meeting Abstract.

Interpretive Summary:

Technical Abstract: Penicillium expansum, one of the patulin producing fungi that causes decay on apple, and is recognized as the main source of patulin on apple and apple products. Currently, liquid-liquid extraction with ethyl acetate followed by high-performance liquid chromatography – ultraviolet detection (HPLC-UV) or liquid chromatography - mass spectroscopy detection (LC-MS) is widely used as a method for patulin analysis in apple juice. In this study, a cyclodextrin polymer developed and confirmed adsorption to patulin in apple juice previously was used for solid phase extraction of patulin in apple juice. Conditions investigated for this method were solvent for column conditioning, sample volume to load on column, solvent for washing, and solvent and volume for patulin elution and optimization. At the optimized conditions, the recovery and reproducibility relative standard deviation (RSDr) of patulin from apple juice at 100, 50, 25, and 10 ng mL-1 were 78 and 3.0, 85 and.3.5, 79 and 11, and 79 and 1.8 % respectively. level of quantitation (LOQ) of patulin in apple juice by this method was 10 ng mL-1.