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ARS Home » Pacific West Area » Corvallis, Oregon » National Clonal Germplasm Repository » Research » Publications at this Location » Publication #285423
Title: Cryopreservation and maintenance of hop material in USDA germplasm collection

Author
item Reed, Barbara
item Hummer, Kim

Submitted to: Acta Horticulture Proceedings
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/1/2012
Publication Date: 1/2/2013
Citation: Reed, B.M., Hummer, K.E. 2013. Cryopreservation and maintenance of hop material in USDA germplasm collection. Acta Horticulture Proceedings. 1010:191-198.

Interpretive Summary: The National Clonal Germplasm Repository (NCGR-Corvallis) is responsible for conservation of the hop (Humulus lupulus L.) genetic resources for the US Department of Agriculture (USDA), Agricultural Research Service (ARS). The collection includes 675 accessions representing 7 Humulus taxa (species and subspecies) from 18 countries. This germplasm includes: wild collected seeds, virus and viroid tested plants growing in greenhouses or screenhouses, in vitro cultures, and cryogenically preserved pollen and meristems. The tissue culture collection is composed of primarily the core collection of pathogen-tested cultivars and wild collected species. The cryopreserved clonal collection was established from tissue-cultured plants and includes 90 accessions stored. These cryopreserved samples are held at the USDA ARS National Center for Genetic Resources Preservation (NCGRP) in Ft. Collins, Colorado. The plants from which NCGR-Corvallis distributes cuttings are tested for known viruses and viroids. Hop germplasm from NCGR-Corvallis is distributed to researchers internationally in accordance with country, regional and state phytosanitary regulations.

Technical Abstract: The National Clonal Germplasm Repository (NCGR-Corvallis) is responsible for conservation of the hop (Humulus lupulus L.) genetic resources for the US Department of Agriculture (USDA), Agricultural Research Service (ARS). The collection includes 675 accessions representing 7 related taxa (species and subspecies) from 18 countries. This germplasm includes: wild collected seeds, virus and viroid tested plants growing in greenhouses or screenhouses, in vitro cultures, and cryogenically preserved pollen and meristems. The tissue culture collection is composed of primarily the core collection of pathogen-tested cultivars and wild collected species. The cryopreserved clonal collection was established from tissue-cultured plants and includes 90 accessions stored using the controlled rate cooling or encapsulation-dehydration techniques. These cryopreserved samples are held at the USDA ARS National Center for Genetic Resources Preservation in Ft. Collins, Colorado. Hop germplasm from NCGR-Corvallis is distributed to researchers internationally in accordance with country, regional and state phytosanitary regulations.