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ARS Home » Midwest Area » Urbana, Illinois » Soybean/maize Germplasm, Pathology, and Genetics Research » Research » Publications at this Location » Publication #287261
Title: Role of Soybean mosaic virus-encoded proteins in seed and aphid transmission in soybean

Author
item JOSSEY, SUSHMA - University Of Illinois
item HOBBS, HOUSTON - University Of Illinois
item Domier, Leslie

Submitted to: Phytopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/2/2013
Publication Date: 9/30/2013
Citation: Jossey, S., Hobbs, H.A., Domier, L.L. 2013. Role of Soybean mosaic virus-encoded proteins in seed and aphid transmission in soybean. Phytopathology. 103(9):941-948.

Interpretive Summary: Soybean mosaic virus (SMV) is a seed and aphid-transmitted virus that can cause significant yield reductions and reduce seed quality in soybean. In North America, seed transmission serves as the primary source of inoculum for SMV. The roles in seed and aphid transmission of SMV encoded proteins were investigated. The results showed that some mutations that reduced transmission of SMV through seed also reduced transmission of SMV by aphids, which suggested that specific interactions between SMV-encoded proteins are important for multiple functions in the virus life cycle. The results of this study will be of interest to scientists who are studying the mechanisms by which viruses are transmitted from plant to plant and from one generation of plants to the next.

Technical Abstract: Soybean mosaic virus (SMV) is seed and aphid transmitted and can cause significant reductions in yield and seed quality in soybean, Glycine max. The roles in seed and aphid transmission of selected SMV-encoded proteins were investigated by constructing chimeric recombinants between SMV 413 (efficiently aphid and seed transmitted) and SMV G2 (not aphid or seed transmitted). As previously reported, the DAG amino acid sequence motif near the amino terminus of the CP was the major determinant in differences in aphid transmissibility of the two SMV isolates, and HC-Pro played a secondary role. Seed transmission of SMV was influenced by P1, HC-Pro, and CP. Replacement of the P1 coding region of SMV 413 with that of SMV G2 significantly enhanced seed transmissibility of SMV 413. Mutation of the G in the DAG motif to D and Q to P near the carboxyl terminus of the CP of SMV 413 significantly reduced seed transmission. The Q to P substitution in SMV 413 also abolished virus-induced seed coat mottling in plant introduction 68671. This is the first report associating P1, CP and the DAG motif with seed transmission of a potyvirus and suggests that HC-Pro interactions with CP are important for multiple functions in the virus life cycle.