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Title: Microsatellite marker development in peony using next generation sequencing

Author
item Gilmore, Barbara
item Bassil, Nahla
item Nyberg, April
item KNAUS, BRIAN - Forest Service (FS)
item SMITH, DON - Yellow Peonies And More
item Barney, Danny
item Hummer, Kim

Submitted to: Journal of the American Society for Horticultural Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/15/2012
Publication Date: 1/20/2013
Citation: Gilmore, B.S., Bassil, N.V., Nyberg, A.M., Knaus, B., Smith, D., Barney, D.L., Hummer, K.E. 2013. Microsatellite marker development in peony using next generation sequencing. Journal of the American Society for Horticultural Science. 138(1):64-74.

Interpretive Summary: Peonies, the grand garden perennial of spring and early summer, are economically important to the international cut flower market. herbaceous peonies, tree peonies , and intersectional crosses between the two types are of interest to gardeners, growers, and nursery producers. Thousands of peony cultivars exist and identity is traditionally determined by experienced horticulturists knowledgeable in plant and bloom characteristics. With DNA extraction possible during any time of the year, molecular markers can provide genotype identity confirmation for dormant roots or mature post bloom-plants. The primary objective of our research was to rapidly and inexpensively develop genetic markers in a range of peony species using DNA sequencing analysis. A secondary objective was to apply these markers to fingerprint 93 accessions that include tree, intersectional and herbaceous peonies. We used 21 markers to distinguish cultivars and their close relatives. Also from our sequence information, > 9000 markers were designed and are made available to other scientists worldwide.

Technical Abstract: Peonies (Paeonia), the grand garden perennial of spring and early summer, are economically important to the international cut flower market. Herbaceous peonies (Paeonia section Paeonia), tree peonies (Paeonia section Moutan), and intersectional crosses between the two types (Itoh Paeonia hybrids) are of interest to gardeners, growers, and nursery producers. Thousands of peony cultivars exist and identity is traditionally determined by experienced horticulturists knowledgeable in plant and bloom characteristics. With DNA extraction possible during any time of the year, molecular markers can provide genotype identity confirmation for dormant roots or mature post bloom-plants. The primary objective of our research was to rapidly and inexpensively develop microsatellite markers in a range of Paeonia species using barcoded Illumina libraries. A secondary objective was to apply these SSR markers to fingerprint 93 accessions that include tree, intersectional and herbaceous peonies. We used 21 primers to distinguish cultivars and their close relatives. Also from our sequence information, > 9000 primers were designed and are made available.