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ARS Home » Pacific West Area » Davis, California » Nat'l Clonal Germplasm Rep - Tree Fruit & Nut Crops & Grapes » Research » Publications at this Location » Publication #288832
Title: Genetic diversity, structure, and patterns of differentiation in the genus Vitis

Author
item Aradhya, Mallikarjuna
item WANG, YUEJIN - Northwest Agriculture And Forestry University
item WALKER, M - University Of California
item Prins, Bernard - Bernie
item Pitcher, Anne
item VELASCO, DIANNE - University Of California
item GERRATH, JEAN - University Of Guelph
item DANGL, GERALD - University Of California
item Preece, John

Submitted to: Plant Systematics and Evolution
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/14/2012
Publication Date: 2/1/2013
Publication URL: http://www.ars-grin.gov/dav
Citation: Aradhya, M.K., Wang, Y., Walker, M.A., Prins, B.H., Pitcher, A.M., Velasco, D., Gerrath, J.M., Dangl, G.S., Preece, J.E. 2012. Genetic diversity, structure, and patterns of differentiation in the genus Vitis. Plant Systematics and Evolution. 299(2):317-330.

Interpretive Summary: Vitis (Vitaceae) is a taxonomically complicated genus with ca. 60 taxa divided into two subgenera, Vitis and Muscadinia. We used population genetic approaches to gain insights into the genetic diversity, patterns of evolutionary differentiation and to decipher the taxonomic status of some of the controversial taxa within the genus Vitis. The distance- and model-based analyses were used to examine the phylogenetic structure within the genus Vitis using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. The results closely matched the current classification, but some discrepancies in the identity of taxa at the specific and subspecific levels were still evident. The East Asian the North American Vitis exhibited strong divergence and each group showed further differentiation into several subgroups with North American subgroups roughly matching the described series. The model based cluster analysis indicated 14 clusters as optimum to explain the genetic structure within the genus Vitis with most clusters containing a moderate frequency of admixed genotypes suggesting interspecific gene flow within the subgenus Vitis. Hierarchical partitioning of molecular variation indicated that a significant amount of total variation (~74% and ~69% for SSRs and AFLPs, respectively) is accounted for by intraspecific variation as compared to the levels due to genetic differentiation among species within series (~17% and ~20% for SSRs and AFLPs, respectively) and among series within the genus Vitis (~9% and ~10% for SSRs and AFLPs, respectively). Overall, Vitis possesses mild genetic structure characterized by reticulation and incomplete lineage sorting of ancestral polymorphisms.

Technical Abstract: Vitis (Vitaceae) is a taxonomically complicated genus with ca. 60 taxa divided into two subgenera, Vitis and Muscadinia. We used population genetic approaches to gain insights into the genetic diversity, patterns of evolutionary differentiation and to decipher the taxonomic status of some of the controversial taxa within the genus Vitis. The distance- and model-based analyses were used to examine the phylogenetic structure within the genus Vitis using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. The results closely matched the current classification, but some discrepancies in the identity of taxa at the specific and subspecific levels were still evident. The East Asian the North American Vitis exhibited strong divergence and each group showed further differentiation into several subgroups with North American subgroups roughly matching the described series. The model based cluster analysis indicated 14 clusters as optimum to explain the genetic structure within the genus Vitis with most clusters containing a moderate frequency of admixed genotypes suggesting interspecific gene flow within the subgenus Vitis. Hierarchical partitioning of molecular variation indicated that a significant amount of total variation (~74% and ~69% for SSRs and AFLPs, respectively) is accounted for by intraspecific variation as compared to the levels due to genetic differentiation among species within series (~17% and ~20% for SSRs and AFLPs, respectively) and among series within the genus Vitis (~9% and ~10% for SSRs and AFLPs, respectively). Overall, Vitis possesses mild genetic structure characterized by reticulation and incomplete lineage sorting of ancestral polymorphisms.