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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Soybean Genomics & Improvement Laboratory » Research » Publications at this Location » Publication #291585
Title: Identification of quantitative trait loci for sucrose content in soybean seed

Author
item ZENG, A - University Of Arkansas
item CHEN, P - University Of Arkansas
item SHI, A - Syngenta Seeds, Inc
item WANG, D - Michigan State University
item ZHANG, B - Virginia State University
item ORAZALY, M - University Of Arkansas
item JAUREGUY, L - University Of Arkansas
item FLOREZ, L - University Of Arkansas
item BRYE, K - University Of Arkansas
item Cregan, Perry
item Song, Qijian

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/1/2015
Publication Date: 5/15/2015
Citation: Zeng, A., Chen, P., Shi, A., Wang, D., Zhang, B., Orazaly, M., Jaureguy, L., Florez, L., Brye, K., Cregan, P.B., Song, Q. 2015. Identification of quantitative trait loci for sucrose content in soybean seed. Crop Science. 54:554-564.

Interpretive Summary: Sucrose is a desirable sugar in soybean seed that affects the quality of soyfoods and feed. Soybean varieties with high sucrose would be valuable for soyfood and meal markets. We analyzed the progeny of a cross between a soybean with high seed sucrose and one with low seed sucrose. The progeny were grown in replicated field trials and the sucrose content in the harvested seed was measured. Both simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) DNA markers were used to analyze the progeny for the purpose of discovering the positions of genes or quantitative trait loci (QTL) that control the level of sucrose in the soybean seed. The analysis resulted in the identification of three QTL on three different soybean chromosomes that control seed sucrose level. The genetic marker information can be used by soybean breeders and geneticists in genetic marker assisted selection to identify soybean breeding lines that will produce seed with high levels of sucrose.

Technical Abstract: Sucrose is a desirable sugar in soybean seed that affects the quality of soyfoods and feed, therefore, soybean cultivars with high sucrose would be valuable for soyfood and meal markets. The objective of this study was to identify quantitative trait loci (QTL) associated with seed sucrose content using simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers that can be used for indirect selection in breeding. A low sucrose line, MFS-553, was crossed with a high sucrose plant introduction, PI 243545, to develop an F2-derived QTL mapping population. A total of 626 SSR primers covering 20 soybean chromosomes were used to screen the parents and 209 SSR markers were polymorphic. Of these, 97 were effective in differentiating the parental alleles in the initial mapping population consisting of 220 F2:3 lines derived from MFS-553 x PI 243545. Subsequently, a total of 94 F3:4 lines derived from the initial F2:3 population were genotyped with 5361 SNP markers spanning 20 chromosomes, of which 2016 were polymorphic. Seed from F2:3, F3:5 and F3:6 lines were analyzed for sucrose using high performance liquid chromatography (HPLC). Following the linkage map construction, composite interval mapping (CIM) and multiple interval mapping (MIM) were performed to locate sucrose QTL. Three novel QTLs for seed sucrose were mapped to chromosomes 5, 9, and 16, accounting for 46%, 10% and 8%, respectively, of the phenotypic variation for sucrose content. SSR and SNP markers associated with these QTLs can be used for marker assisted selection in breeding soybean lines with a desired sugar profile.