Detection of Fusarium oxysporum f. sp. vasinfectum race 3 by single-base extension method and allele-specific polymerase chain reaction

Authors: EMGAMBERDIEV, SHAROF - Uzbekistan Academy Of Sciences; SALAKHUDINOV, ILKHOM - Uzbekistan Academy Of Sciences; ABDULLAEV, ALISHER - Uzbekistan Academy Of Sciences; Ulloa, Mauricio; Saha, Sukumar; RADJAPOV, FARHOD - Uzbekistan Academy Of Sciences; MULLAOHUNOV, BAHTIYOR - Uzbekistan Academy Of Sciences; MANSUROV, DAMIR - Uzbekistan Academy Of Sciences; Jenkins, Johnie; ABDURAKHMONOV, IBROKHIM - Uzbekistan Academy Of Sciences

Submitted to: Canadian Journal of Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/6/2014
Publication Date: 7/7/2014
Citation: Emgamberdiev, S.S., Salakhudinov, I.B., Abdullaev, A., Ulloa, M., Saha, S., Radjapov, F., Mullaohunov, B., Mansurov, D., Jenkins, J.N., Abdurakhmonov, I.Y. 2014. Detection of Fusarium oxysporum f. sp. vasinfectum race 3 by single-base extension method and allele-specific polymerase chain reaction. Canadian Journal of Plant Pathology. 36(2):216-223.

Interpretive Summary: Fusarium wilt is an important disease caused by a fungus [Fusarium oxysporum f.sp vasinfectum (FOV]. This disease reduces yield in cotton and many other crops. The cotton host-specific genotypes called races significantly differ in the way they affect and infect cotton cultivars. Herein, we report for the first time, allele specific (AS) single nucleotide polymorphism-markers developed for a rapid detection of FOV race 3, using single-base extension method and allele-specific polymerase chain reaction. The assay is useful for specific and rapid detection of Fusarium oxysporum f.sp vasinfectum race 3. These markers will help breeders to develop strategies to combat the Fusarium wilt disease by breeding resistant cotton cultivars in Uzbekistan and will help in predicting and preventing plant disease outbreaks.

Technical Abstract: We developed allele specific (AS) SNP primers for rapid detection of Fusarium oxysporum f.sp vasinfectum (FOV) race 3. FOV_BT_SNP_R3 and FOV_BT_AS_R3 primers were designed based on single nucleotide polymorphisms of partial sequence alignment of the ß-tubulin (BT) gene from several FOV races. These primers showed high specificity for FOV race 3, and exclusively amplified PCR-products from FOV race 3 only. No amplification products were detected for races 1, 2, 4, and 8 FOV genotypes using these primers. The assay is useful for specific and rapid detection of Fusarium oxysporum f.sp vasinfectum race 3.