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Title: Molecular and phytochemical investigation of Angelica dahurica and Angelica pubescentis essential oils and their biological activity against Aedes aegypti, Stephanitis pyrioides and Colletotrichum species

Author
item TABANCA, NURHAYAT - University Of Mississippi
item GAO, ZENGPING - Beijing University Of Chinese Medicine
item DEMIRCI, BETUL - Anadolu Universtiy
item TECHEN, NATASCHA - University Of Mississippi
item Wedge, David
item ALI, ABBAS - University Of Mississippi
item Sampson, Blair
item Werle, Christopher
item Bernier, Ulrich
item KHAN, IKHLAS - University Of Mississippi
item CAN BASER, KEMAL - King Saud University

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/18/2014
Publication Date: 8/18/2014
Publication URL: https://handle.nal.usda.gov/10113/4861473
Citation: Tabanca, N., Gao, Z., Demirci, B., Techen, N., Wedge, D.E., Ali, A., Sampson, B.J., Werle, C.T., Bernier, U.R., Khan, I.A., Can Baser, K.H. 2014. Molecular and phytochemical investigation of Angelica dahurica and Aneelica pubescentis essential oils and their biological activity against Aedes aegypti, Stephanitis pyrioides and Colletotrichum species. Journal of Agricultural and Food Chemistry. 62:8848-8857.
DOI: https://doi.org/10.1021/jf5024752

Interpretive Summary: Water distilled essential oils from the roots of Angelica dahurica and Angelica pubescentis were investigated for their antifungal activity against plant pathogens Colletotrichum acutatum, C. fragariae, and C. gloeosporioides as well as insecticidal activity against the yellow fever mosquito, Aedes aegypti and azalea lace bugs, Stephanitis pyrioides. Water distilled essential oils from the roots of Angelica dahurica and Angelica pubescentis were investigated for their antifungal activity against plant pathogens Colletotrichum acutatum, C. fragariae, and C. gloeosporioides as well as insecticidal activity against the yellow fever mosquito, Aedes aegypti and azalea lace bugs, Stephanitis pyrioides. Forty-three compounds, representing 92.1% of the A. dahurica total essential oil and 46 components, which made up 86.2% of the A. pubescentis total essential oil, were characterized. A. pubescentis root essential oil demonstrated moderate antifungal activity against Colletotrichum species, whereas A. dahurica root essential oil did not show antifungal activity. Conversely, A. dahurica root essential oil demonstrated deterrent and insecticidal activity against Ae. aegypti and azalea lace bugs, S. pyrioides. Chemical diversity appears to play a role in determining the antifungal and insecticidal activities of essential oils. Chemical diversity appears to play a role in determining the antifungal and insecticidal activities of essential oils.

Technical Abstract: Water distilled essential oils from the roots of Angelica dahurica and Angelica pubescentis were investigated for their antifungal activity against plant pathogens Colletotrichum acutatum, C. fragariae, and C. gloeosporioides as well as insecticidal activity against the yellow fever mosquito, Aedes aegypti and azalea lace bugs, Stephanitis pyrioides. Forty-three compounds, representing 92.1% of the A. dahurica total essential oil and 46 components, which made up 86.2% of the A. pubescentis total essential oil, were characterized. The major compounds in the A. dahurica oil were found to be betapinene (46.3%), sabinene (9.3%), myrcene (5.5%), 1-dodecanol (5.2%) and terpinen-4-ol (4.9%), beta-Pinene (37.6%), p-cymene (11.6%), limonene (8.7%) and cryptone (6.7%) were the primary compounds found in the A. pubescentis oil. A. pubescentis root essential oil demonstrated moderate antifungal activity against Colletotrichum species, whereas A. dahurica root essential oil did not show antifungal activity. Conversely, A. dahurica root essential oil demonstrated deterrent and insecticidal activity against Ae. aegypti and azalea lace bugs, S. pyrioides. In mosquito bioassays, 1-dodecanol and 1-tridecanol showed anti-biting deterrent activity similar to the positive control DEET (N,N-diethyl-3-methylbenzamide) at 25 nmol/cm2 against Aedes aegypti, whereas only 1-tridecanol showed repellent activity in human based cloth patch bioassay with minimum effective dosages (MED) of 0.086 ± 0.089 mg/cm2 (DEET: 0.007 ± 0.003 mg/cm2). In larval bioassays, 1-tridecanol was more toxic with LD50 value of 2.1 ppm than 1-dodecanol having LD50 value of 5.2 ppm against 1-day old Ae. aegypti larvae. Chemical diversity appears to play a role in determining the antifungal and insecticidal activities of essential oils. 1-Dodecanol and 1-tridecanol could be useful for the natural mosquito control agents. These two Angelica species were also analyzed genetically using the nuclear ribosomal internal transcribed spacer (ITS) region as a DNA barcode marker.