Author
![]() |
MALMIERCA, M - University Of Leon |
![]() |
CARDOZA, R - University Of Leon |
![]() |
McCormick, Susan |
![]() |
Alexander, Nancy |
![]() |
BARREIRO, C - Inbiotec |
![]() |
MONTE, E - University Of Salamanca |
![]() |
GUTIERREZ, S - University Of Leon |
Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 10/8/2014 Publication Date: 10/8/2014 Citation: Malmierca, M.G., Cardoza, R.E., McCormick, S.P., Alexander, N.J., Barreiro, C., Monte, E., Gutierrez, S. 2014. Proteomic analysis of a mutant of Trichoderma arundinaceum impaired in the trichothecene biosynthesis reveals a systemic function of these compounds in the fungal physiology [abstract]. Human Proteome Organization World Congress. Interpretive Summary: Technical Abstract: Trichothecenes are sesquiterpene mycotoxins produced by several fungal genera including Fusarium, Trichothecium, Myrothecium, Stachybotrys, and Trichoderma. These toxins have attracted great attention because they are frequent contaminants of food and animal feed, and can be easily absorbed by animals and human beings via the integumentary and gastrointestinal systems. Exposure to these toxins can cause feed refusal, immunological problems, vomiting, skin dermatitis, and hemorrhagic lesions. Trichoderma trichothecenes have been studied as model compounds since they are relatively structurally simpler compounds, and their biosynthesis requires relatively fewer genes, in comparison with trichothecenes produced by other genera. Most of the Trichoderma trichothecene genes are within a cluster but the sesquiterpene cyclase gene tri5 is located in a different genomic region. Tri5 controls the first specific step in trichothecene biosynthesis, cyclization of farnesyl diphosphate (FPP) to trichodiene. In the present work we analyzed the proteome changes produced in Ta'Tri5, a trichothecene non-producing tri5 mutant, in comparison with the wild-type strain, Trichoderma arundinaceum IBT 40837. Sixty differentially expressed spots were detected with 2D-DIGE analysis and identified with MALDI TOF-TOF. Among them, 21 spots were differentially overproduced in Ta'Tri5 and the other 39 were differentially overproduced in the Trichoderma arundinaceum wild-type strain. The differences suggest that genetic disruption of tri5 had a systemic effect of the fungal physiology, including changes in regulation of intracellular FPP and acetyl CoA levels. |